Recombinant Mouse CD83 Fc Chimera Protein, CF

Catalog #: 1437-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1437-CD-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD83 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of human monocyte-derived dendritic cells. When 5 x 10⁴ cells/well are added to mouse CD83/Fc Chimera coated plates (2.5 µg/mL with 100 µL/well), approximately 50-75% will adhere after 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse CD83 protein

Mouse CD83 (Met22 - Ala134) Accession #NP_033986	DIEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

NP_033986

N-terminal Sequence
Analysis

Met22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

39.1 kDa (monomer)

SDS-PAGE

52 kDa, reducing conditions

Product Datasheets

1437-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1437-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD83

Mouse CD83 is a 30 - 35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45 - 55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8⁺ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4⁺ T cell phenotype (15).

References

Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
Scholler, N. et. al. (2001) J. Immunol. 166:3865.
Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
Hock, B.D. et al. (2001) Int. Immunol. 13:959.
Dudziak, D. et al. (2005) J. Immunol. 174:6672.
Kotzor, N. et al. (2004) Immunobiology 209:129.
Zinser, E. et al. (2006) Immunobiology 21:449.
Hirano, N. et al. (2006) Blood 107:1528.
Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
Yamashiro, S. et al. (2000) Blood 96:3958.
Cao, W. et al. (2005) Biochem. J. 385:85.
Scholler, N. et al. (2002) J. Immunol. 168:2599.
Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Entrez Gene IDs

9308 (Human); 12522 (Mouse)

Alternate Names

B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83