Recombinant Mouse CDNF Protein, CF Summary
Product Specifications
Leu27-Leu187, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5187-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: CDNF
CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1), is a 17 - 19 kDa secreted protein that shares 52% amino acid (aa) identity with mouse MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet (1). The Armet designation is not preferred, because the proteins as translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfide bonds, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1 - 3). The mouse CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature mouse CDNF shares 80%, 87%, 83% and 82% aa identity with human, rat, equine and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre and postnatal mouse brain, they are mostly abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson's disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).
- Lindholm, P. et al. (2007) Nature 448:73.
- Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
- Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
- Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.
Citations for Recombinant Mouse CDNF Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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CDNF Interacts with ER Chaperones and Requires UPR Sensors to Promote Neuronal Survival
Authors: A Eesmaa, LY Yu, H Göös, T Danilova, K Nõges, E Pakarinen, M Varjosalo, M Lindahl, P Lindholm, M Saarma
International Journal of Molecular Sciences, 2022-08-22;23(16):.
Species: N/A
Sample Types: N/A
Applications: ELISA Standard -
Characterization of CDNF-Secreting ARPE-19 Cell Clones for Encapsulated Cell Therapy
Authors: E Galli, P Lindholm, LS Kontturi, M Saarma, A Urtti, M Yliperttul
Cell Transplant, 2019-03-06;28(4):413-424.
Species: Human
Sample Types: Protein
Applications: ELISA Control
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