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Recombinant Mouse CLEC-2/CLEC1B Protein, CF

Catalog #: 9117-CL Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9117-CL-050
Recombinant Mouse CLEC-2/CLEC1B Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CLEC-2/CLEC1B Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL) is coated at 1.0 µg/mL (100 μL/well), Recombinant Mouse CLEC‑2/CLEC1B binds with a typical ED50 of 0.1-0.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CLEC-2/CLEC1B protein
Met53-Leu229, with an N-terminal 6-His tag
Accession #
Q9JL99
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
21 kDa
SDS-PAGE
24-39 kDa, reducing conditions

Product Datasheets

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9117-CL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9117-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse CLEC-2/CLEC1B Protein Bioactivity View Larger

When Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL) is coated at 1.0 µg/mL, Recombinant Mouse CLEC-2/CLEC1B (Catalog # 9117-CL) binds with a typical ED50 of 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2, also known as CLEC-1B, is a 32 kDa type II transmembrane glycoprotein that is expressed on platelets, megakaryocyte, and liver sinusoidal cells (1-4). Mature mouse CLEC-2 consists of a 31 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) with one C-type lectin domain. Within the ECD, mouse CLEC-2 shares 59% and 90% aa sequence identity with human and rat CLEC-2, respectively. Alternative splicing generates a short isoform that lacks the transmembrane segment. CLEC-2 binding to Podoplanin trigger platelet activation, shedding of GPVI, and cleavage of Fcg RIIA/CD32a (5-8). CLEC-2 expression on platelets is required for the development of lymphatic vessels and valves as well as the maintenance of lymph node high endothelial veinules (HEV) during lymphocyte crossing (7-10). CLEC-2 additionally binds to fucoidan, HIV-1, and the platelet-aggregating snake venom protein Rhodocytin/Aggretin (2, 3, 11). In the liver, soluble CLEC-2 promotes M2 macrophage differentiation and limits inflammatory cytokine production (12).

References
  1. Lee, R.H. and W. Bergmeier (2016) J. Thromb. Haemost. Epub PMID 26749528.
  2. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  3. Chaipan, C. et al. (2006) J. Virol. 80:8951.
  4. Gitz, E. et al. (2014) Blood 124:2262.
  5. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  6. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  7. Herzog, B.H. et al. (2013) Nature 502:105.
  8. Sweet, D.T. et al. (2015) J. Clin. Invest. 125:2995.
  9. Hess, P.R. et al. (2014) J. Clin. Invest. 124:273.
  10. Osada, M. et al. (2012) J. Biol. Chem. 287:22241.
  11. Manne, B.K. et al. (2013) J. Biol. Chem. 288:7717.
  12. Wu, X. et al. (2015) EbioMedicine 2:214.
Long Name
C-type Lectin-like Receptor 2
Entrez Gene IDs
51266 (Human); 56760 (Mouse)
Alternate Names
1810061I13Rik; CLEC1B; CLEC2; CLEC-2; CLEC2B; CLEC2PRO1384; C-type lectin domain family 1 member B; C-type lectin domain family 1, member B; C-type lectin-like receptor 2; C-type lectin-like receptor-2; QDED721

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