Recombinant Mouse COMP Protein, CF

Name: Recombinant Mouse COMP Protein, CF
Brand: R&D Systems
SKU: 8958-CP
Rating: 5 (1 reviews)

Catalog #: 8958-CP 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8958-CP-050

Recombinant Mouse COMP Protein Binding Activity

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All COMP/Thrombospondin-5 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse COMP Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.04-0.6 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse COMP/Thrombospondin-5 protein
Gln20-Val755, with a C-terminal 6-His tag

Accession #

Q9R0G6

N-terminal Sequence
Analysis

No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Gly21

Structure / Form

Disulfide-linked pentamer

Predicted Molecular Mass

81 kDa

SDS-PAGE

85-115 kDa, reducing conditions

Product Datasheets

8958-CP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8958-CP

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Mouse COMP/Thrombospondin-5 (Catalog # 8958-CP) induces adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.15-0.9 μg/mL.

Reconstitution Calculator

Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). Mouse COMP contains a non-collagenous coiled-coil domain, four EGF-like repeats, eight TSP type-3 repeats, and a globular TSP C-terminal domain (5). It shares 92% and 98% aa sequence identity with human and rat COMP, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D₃ which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is up-regulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).

References

Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
Posey, K.L. et al. (2014) Matrix Biol. 37:167.
Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
Fang, C. et al. (2000) J. Orthop. Res. 18:593.
DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
Ozbek, S., et al. (2002) EMBO J. 21:5960.
Chen, F.H., et al. (2005) J. Biol. Chem. 280:32655.
Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
Kennedy, J., et al. (2005) Eur. J. Hum. Genet. 13:547.
Hou, J. et al. (2000) Cell Calcium 27:309.

Long Name

Cartilage Oligomeric Matrix Protein

Entrez Gene IDs

1311 (Human); 12845 (Mouse)

Alternate Names

cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5