Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF

Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CTRP4/C1qTNF4

C1qTNF4, also known as CTRP4, is an approximately 34 kDa member of the C1q family of secreted proteins (1, 2). C1qTNF4 consists of a signal peptide followed by two tandem globular C1q domains (1). It is the only C1q family member with tandem C1q domains (1, 2). The second C1q domain contains an RGD peptide, suggesting that it could potentially interact with integrins (3). Mature mouse C1qTNF4 shares 95% and 98% amino acid sequence identity with human and rat C1qTNF4, respectively. Expression of human C1qTNF4 occurs most abundantly in brain and adipose tissue (1). Neurons in the hypothalamus express and secrete CTRP4 which modulates food intake and energy balance by controlling neuropeptide gene expression (1). In-house finding shows CTRP4 can support neurite outgrowth of cortical neurons. Like other CTRP family members, C1qTNF4 can form dimers, trimers, hexamers, and high molecular weight oligomers following secretion (1, 4). Injection of recombinant C1qTNF4 into mice can reduce food intake, body weight, and ambulatory activity levels (1). C1qTNF4 has also been shown to promote human cancer cell survival in vitro (5).

Related Research Areas

• Adipocytokines
• Complement Pathway
• Glucose Homeostasis
• Obesity
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