Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF Summary
Product Specifications
| Mouse C1qTNF9 (Gln20-Ser333) Accession # Q4ZJN1 | HPGGGSGGGSGGGS | HHHHHH |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2132-TN
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose. |
| Reconstitution | Reconstitute at 250 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
When Recombinant Mouse CTRP9/C1qTNF9 (Catalog # 2132-TN) is coated at 0.5 μg/mL, 100 μL/well, Biotinylated Recombinant Human CL-K1/COLEC11 binds with an ED50 of 15-90 ng/mL.
View Larger
2 μg/lane of Recombinant Mouse CTRP9/C1qTNF9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-43 kDa and 39-43 & 80-90 kDa, respectively.
Reconstitution Calculator
Background: CTRP9/C1qTNF9
C1qTNF9, also known as CTRP9, is an approximately 40 kDa member of the C1q and TNF-related protein family (1). Like all members of this protein family, C1qTNF9 consists of a short variable region, a collagenous domain that can be hydroxylated, and a C1q-like globular domain (1). Mouse C1qTNF9 shares 86% and 98% amino acid sequence identity with the human and rat orthologs, respectively. Both the mouse and human C1qTNF9 proteins are expressed mostly in adipose tissue, but the mouse protein has also been detected in the heart, lung, muscle, kidney, testis, lymph node, smooth muscle, prostate, thymus, and uterus (1, 2). C1qTNF9 has been shown to be secreted as trimers and higher order multimers and also to form hetero-oligomers with Adiponectin (1, 2). Mouse C1qTNF9 can stimulate the phosphorylation of AMPK, Akt, and eNOS (1, 3, 4). Also in mice, C1qTNF9 may have an important role in cardiac and metabolic health. Its expression has a cardioprotective effect following acute myocardial infarction that may be dependent on AMPK activation (4-6). Additionally, transgenic mice overexpressing C1qTNF9 are resistant to high fat diet‑induced obesity (7). This metabolic role may be conserved, since C1qTNF9 serum levels have been shown to inversely correlate with metabolic syndrome in humans (8).
- Wong, G.W. et al. (2009) FASEB J. 23:241.
- Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
- Zheng, Q. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:2616.
- Kambara, T. et al. (2012) J. Biol. Chem. 287:18965.
- Su, H. et al. (2013) Basic Res. Cardiol. 108:315.
- Sun, Y. et al. (2013) Circulation 128:S113.
- Peterson, J.M. et al. (2013) Am. J. Physiol. Regul. Integr. Comp. Physiol. 305: R522.
- Hwang, Y.C. et al. (2014) Int. J. Obes. (Lond) 38:1207
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF and earn rewards!
Have you used Recombinant Mouse CTRP9/C1qTNF9 His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
