Home / Decorin / Recombinant Mouse Decorin Protein, CF

Recombinant Mouse Decorin Protein, CF

Catalog #: 1060-DE
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1060-DE-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (12)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Decorin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. 5 µg/mL of Recombinant Mouse Decorin can significantly delay the rate of collagen fibrillogenesis.
Source
Mouse myeloma cell line, NS0-derived mouse Decorin protein
Gly17-Lys354 & Asp31-Lys354, with a C-terminal 10-His tag
Accession #
P28654
N-terminal Sequence
Analysis
Gly17 & Asp31
Predicted Molecular Mass
39 kDa
SDS-PAGE
40-45 kDa, reducing conditions

Product Datasheets

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1060-DE

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1060-DE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Decorin

Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10 ‑ 12 tandem leucine-rich repeats (LRR) (1). The mouse Decorin cDNA encodes a 354 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide (2). Mature mouse Decorin contains twelve tandem LRR and shares 80% and 87% aa sequence identity with human and rat Decorin, respectively. Decorin is an N-glycosylated protein that also carries a variably-sized hybrid chondroitin/dermatan sulfate chain at Ser34 (2, 3). Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa (4). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF-2, GDF-8/Myostatin, TGF-beta, and WISP-1 (3, 5 ‑ 8). It also binds and activates EGF R, ErbB4, and IGF-I R (9 ‑ 11). In vivo, Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression (12 ‑ 15). Decorin is cleared from the extracellular space by LRP‑mediated endocytosis (16).

References
  1. Schaefer, L. and R.V. Iozzo (2008) J. Biol. Chem. 283:21305.
  2. Scholzen, T. et al. (1994) J. Biol. Chem. 269:28270.
  3. Zamfir, A. et al. (2003) Glycobiology 13:733.
  4. Roughley, P.J. and R.J. White (1989) Biochem. J. 262:823.
  5. Ferdous, Z. et al. (2007) J. Biol. Chem. 282:35887.
  6. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
  7. Cabello-Verrugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
  8. Desnoyers, L. et al. (2001) J. Biol. Chem. 276:47599.
  9. Zhu, J.-X. et al. (2005) J. Biol. Chem. 280:32468.
  10. Santra, M. et al. (2000) J. Biol. Chem. 275:35153.
  11. Schonherr, E. et al. (2005) J. Biol. Chem. 280:15767.
  12. Olguin, H.C. et al. (2003) Dev. Biol. 259:209.
  13. Schonherr, E. et al. (2004) J. Vasc. Res. 41:499.
  14. Seidler, D.G. et al. (2006) J. Biol. Chem. 281:26408.
  15. Reed, C.C. et al. (2005) Oncogene 24:1104.
  16. Brandan, E. et al. (2006) J. Biol. Chem. 281:31562.
Entrez Gene IDs
1634 (Human); 13179 (Mouse)
Alternate Names
Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

Citations for Recombinant Mouse Decorin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Brain injury drives optic glioma formation through neuron-glia signaling
    Authors: Chatterjee, J;Koleske, JP;Chao, A;Sauerbeck, AD;Chen, JK;Qi, X;Ouyang, M;Boggs, LG;Idate, R;Marco Y Marquez, LI;Kummer, TT;Gutmann, DH;
    Acta neuropathologica communications
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  2. A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs
    Authors: Wang, J;Ocadiz-Ruiz, R;Hall, MS;Bushnell, GG;Orbach, SM;Decker, JT;Raghani, RM;Zhang, Y;Morris, AH;Jeruss, JS;Shea, LD;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Abeta amyloidosis
    Authors: R Jiang, U Smailovic, H Haytural, BM Tijms, H Li, RM Haret, G Shevchenko, G Chen, A Abelein, J Gobom, S Frykman, M Sekiguchi, R Fujioka, N Watamura, H Sasaguri, S Nyström, P Hammarströ, TC Saido, V Jelic, S Syvänen, H Zetterberg, B Winblad, J Bergquist, PJ Visser, P Nilsson
    Acta neuropathologica communications, 2022-07-04;10(1):96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. DCN released from ferroptotic cells ignites AGER-dependent immune responses
    Authors: J Liu, S Zhu, L Zeng, J Li, DJ Klionsky, G Kroemer, J Jiang, D Tang, R Kang
    Autophagy, 2021-12-29;0(0):1-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
    Authors: J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann
    Nature Communications, 2021-12-08;12(1):7122.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  6. Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly
    Authors: AC Daquinag, Z Gao, C Fussell, K Sun, MG Kolonin
    Cells, 2020-12-09;9(12):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. Binding of omeprazole to protein targets identified by monoclonal antibodies
    Authors: NMP Cartee, MM Wang
    PLoS ONE, 2020-09-18;15(9):e0239464.
    Species: Mouse
    Sample Types: Reference Standard
  8. Mineralocorticoid receptor antagonism limits experimental choroidal neovascularization and structural changes associated with neovascular age-related macular degeneration
    Authors: M Zhao, I Mantel, E Gelize, X Li, X Xie, A Arboleda, M Seminel, R Levy-Boukr, M Dernigogho, A Prunotto, C Andrieu-So, C Rivolta, J Canonica, MC Naud, S Lechner, N Farman, I Bravo-Osun, R Herrero-Va, F Jaisser, F Behar-Cohe
    Nat Commun, 2019-01-21;10(1):369.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  9. Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells
    Authors: AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin
    Mol Ther Oncolytics, 2017-05-17;6(0):1-9.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  10. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012-10-07;491(7426):765-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
    Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
    Blood, 2011-11-23;119(7):1683-92.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells.
    Authors: Daquinag AC, Zhang Y, Amaya-Manzanares F, Simmons PJ, Kolonin MG
    Cell Stem Cell, 2011-06-16;9(1):74-86.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Binding Assay

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Recombinant Mouse Decorin Protein, CF
By Anonymous on 05/19/2016
Application: In vitro bioactivity in cell culture