Recombinant Mouse Desmoglein-2 Fc Chimera Protein, CF Summary
Product Specifications
Optimal dilutions should be determinded by each laboratory for each application.
| Mouse Desmoglein-2 (Leu29-Ala618) Accession # O55111 |
IEGRMDP | Mouse IgG2A (Gln98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7699-DM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Desmoglein-2
Desmoglein-2 (DSG2) is a 160 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. It is a major protein component of desmosomal contacts between epithelial cells (1, 2). Mature mouse Desmoglein‑2 consists of a 564 amino acid (aa) extracellular domain (ECD) with four cadherin domains, a 21 aa transmembrane segment, and a 483 aa cytoplasmic domain with six desmoglein repeats (3, 4). Within the ECD, mouse Desmoglein‑2 shares 81% and 93% aa sequence identity with human and rat Desmoglein‑2, respectively. A 60 kDa ECD fragment can be proteolytically shed, and this is enhanced by EGF R activation (5‑7). The cytoplasmic domain is cleaved during apoptosis, leaving the transmembrane segment and ECD as a cell‑associated fragment (8). Desmoglein‑2 is widely expressed in epithelia and is required for maintenance of the epithelial barrier (1, 2, 9). It can be either up- or down‑regulated in a variety of carcinomas during tumor progression (10). Desmoglein‑2 also functions as a cell adhesion receptor for several adenovirus serotypes (11). This binding disrupts intercellular junctions between epithelial cells and promotes epithelial‑mesenchymal transition (EMT) (11). Mutations in Desmoglein‑2 cause cardiac dysfunction typical of arrythmogenic right ventricular cardiomyopathy (ARVC) (12).
- Brooke, M.A. et al. (2012) J. Pathol. 226:158.
- Thomason, H.A. et al. (2010) Biochem. J. 429:419.
- Mahoney, M.G. et al. (2002) Exp. Dermatol. 11:115.
- Whittock, N.V. (2003) J. Invest. Dermatol. 120:970.
- Bech-Serra, J.J. et al. (2006) Mol. Cell. Biol. 26:5086.
- Klessner, J.L. et al. (2009) Mol. Biol. Cell 20:328.
- Ramani, V.C. et al. (2008) BMC Cancer 8:373.
- Nava, P. et al. (2007) Mol. Biol. Cell 18:4565.
- Schlegel, N. et al. (2010) Am. J. Physiol. Gastrointest. Liver Physiol. 298:G774.
- Dusek, R.L. and L.D. Attardi (2011) Nat. Rev. Cancer 11:317.
- Wang, H. et al. (2011) Nat. Med. 17:96.
- Pilichou, K. et al. (2009) J. Exp. Med. 206:1787.
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