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Recombinant Mouse EGF-L6 Protein, CF

Catalog #: 4329-EG Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4329-EG-025
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Recombinant Mouse EGF-L6 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Osada, A. et al. (2005) Exp. Cell Res. 303:148. Recombinant Mouse EGF-L6 immobilized at 2 µg/mL, 100 µL/well, will mediate >60% NIH/3T3 cell adhesion (100 µL/well at 5 x 105 cells/mL).
Source
Mouse myeloma cell line, NS0-derived mouse EGF-L6 protein
Thr287-Gly550 & Lys291-Gly550, both with a C-terminal 6-His tag
Accession #
Q9JJZ5
N-terminal Sequence
Analysis
Thr287 & Lys291
Predicted Molecular Mass
30 kDa
SDS-PAGE
37-40 kDa, reducing conditions

Product Datasheets

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4329-EG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4329-EG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF-L6

Epidermal Growth Factor-like Domain, Multiple 6 (EGF-L6), also known as MAM- and EGF-containing (MAEG) is a member of the EGF repeat superfamily of proteins. This secreted protein contains five EGF-like repeats, an RGD motif that binds to Integrin alpha 8 beta 1, and a C-terminal MAM domain (1, 2). Within shared regions, mouse EGF-L6 shares 75% and 91% amino acid sequence identity with the human and rat orthologs, respectively. EGF-L6 is expressed in a multitude of fetal tissues during early development such as lung, heart, liver, spleen, cochlea, and placenta (3, 4). It is also expressed in the dermatome and dermatome-derived tissues including trunk dermis, hair follicles, and cranio-facial mesenchyme (5). EGF-L6 is expressed by osteoblastic-like cells and has been shown to act in a paracrine manner to promote migration of vascular endothelial cells, thus regulating angiogenesis in the local environment of the bone (4, 6). Additionally, EGF-L6 is expressed by epidermal stem cells in the hair follicle bulge and is believed to play a role in regulating the dermal vasculature (7). EGF-L6 is also produced by mature adipocytes and is thought to be involved in the process of adipose tissue expansion and the development of obesity (8). EGF-L6 expression is increased in fibroblastic meningioma and ovarian cancer and has been proposed as a biomarker for the latter (9, 10).

References
  1. Buchner, G. et al. (2000) Genomics 65:16.
  2. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
  3. Yeung, G. et al. (1999) Genomics 62:304.
  4. Chim, S.M. et al. (2011) J. Biol. Chem. 286:22035.
  5. Buchner, G. et al. (2000) Mech. Dev. 98:179.
  6. Chim, S.M. et al. (2013) Cytokine Growth Factor Rev. 24:297.
  7. Xiao, Y. et al. (2013) J. Invest. Dermatol. 133:2324.
  8. Oberauer, R. et al. (2010) Mol. Cell. Biochem. 343:257.
  9. Buckanovich, R.J. et al. (2007) J. Clin. Oncol. 25:852.
  10. Wang, X. et al. (2012) PLoS ONE 7:e52707.
Long Name
EGF-like Domain, Multiple 6
Entrez Gene IDs
25975 (Human); 54156 (Mouse); 317470 (Rat)
Alternate Names
DKFZp564P2063; EGF repeat-containing protein 6; EGFL6; EGF-L6; EGF-like protein 6; EGF-like-domain, multiple 6; epidermal growth factor-like protein 6; MAEG; MAM and EGF domain containing; MAM and EGF domains-containing gene protein; W80

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