Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Mouse Ephrin-B2 (Arg27-Ala227) Accession # AAA82934 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
496-EB
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ephrin-B2
Ephrin‑B2, also known as Htk‑L, ELF‑2, LERK‑5, and NLERK‑1, is a 40 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B2 preferentially interacts with receptors in the EphB family (1, 2). Mature mouse Ephrin‑B2 consists of a 204 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain [Cerretti 1197, Bergemann 4921, Bennett 1866]. Within the ECD, mouse Ephrin‑B2 shares 97% and 98% aa sequence identity with human and rat Ephrin‑B2, respectively. Ephrin‑B2 is expressed presynaptically on neurons (6, 7). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (6‑8). Ephrin‑B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (9, 10). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (8‑11). Ephrin‑B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (12, 13). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (14‑16). It functions as a cellular entry receptor for Hendra and Nipah viruses (17). Ephrin‑B2 is also important for the separation of the urinary and intestinal tracts during development (18).
- Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
- Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
- Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
- Bergemann, A.D. et al. (1995) Mol. Cell. Biol. 15:4921.
- Bennett, B.D. et al. (1995) Proc. Natl. Acad. Sci. 92:1866.
- McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
- Zhao, J. et al. (2010) Mol. Pain 6:77.
- Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
- Foo, S.S. et al. (2006) Cell 124:161.
- Wang, Y. et al. (2010) Nature 465:483.
- Sawamiphak, S. et al. (2010) Nature 465:487.
- Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
- Yu, G. et al. (2003) J. Immunol. 171:106.
- Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
- Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
- Liu, W. et al. (2004) Br. J. Cancer 90:1620.
- Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
- Dravis, C. et al. (2004) Dev. Biol. 271:272.
Citations for Recombinant Mouse Ephrin-B2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC
Authors: Abdelazeem, KNM;Nguyen, D;Corbo, S;Darragh, LB;Matsumoto, MW;Van Court, B;Neupert, B;Yu, J;Olimpo, NA;Osborne, DG;Gadwa, J;Ross, RB;Nguyen, A;Bhatia, S;Kapoor, M;Friedman, RS;Jacobelli, J;Saviola, AJ;Knitz, MW;Pasquale, EB;Karam, SD;
Oncogene
Species: Human
Sample Types: Whole Cells, Transfected Whole Cells
Applications: Bioassay -
Activation of EphrinB2/EphB2 signaling in the spine cord alters glia-neuron interactions in mice with visceral hyperalgesia following maternal separation
Authors: Guo, S;Wang, Y;Duan, Q;Gu, W;Fu, Q;Ma, Z;Ruan, J;
Frontiers in pharmacology
Species: Mouse
Sample Types: In Vivo
Applications: In vivo assay -
Loss of Zmiz1 in mice leads to impaired cortical development and autistic-like behaviors
Authors: K C, R;Patel, NR;Thurmon, AN;Lorino, MG;Tiemroth, AS;Kulstad, I;Morrison, V;Akumuo, M;Shenoy, A;Meadows, SM;Galazo, MJ;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function
Authors: Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A;
bioRxiv : the preprint server for biology
Species: Avian - Chicken, Human
Sample Types: Transfected Whole Cells, Whole Tissue
Applications: Bioassay -
Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays
Authors: M Yang, W Zhu, T Truong, B Pickering, S Babiuk, D Kobasa, L Banadyga
Viruses, 2022-07-28;14(8):.
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Capture -
EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment
Authors: S Bhatia, D Nguyen, LB Darragh, B Van Court, J Sharma, MW Knitz, M Piper, S Bukkapatna, J Gadwa, TE Bickett, S Bhuvane, S Corbo, B Wu, Y Lee, M Fujita, M Joshi, LE Heasley, RL Ferris, O Rodriguez, C Albanese, M Kapoor, EB Pasquale, SD Karam
Nature Communications, 2022-06-20;13(1):3535.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Novel Roles of the Nipah Virus Attachment Glycoprotein and Its Mobility in Early and Late Membrane Fusion Steps
Authors: V Ortega, JLR Zamora, IA Monreal, DT Hoffman, S Ezzatpour, GP Johnston, EM Contreras, FJ Vilchez-De, HC Aguilar
MBio, 2022-05-04;0(0):e0322221.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay -
Angiogenesis depends upon EPHB4-mediated export of collagen IV from vascular endothelial cells
Authors: D Chen, ED Hughes, TL Saunders, J Wu, MN Hernández, T Makinen, PD King
JCI Insight, 2022-02-22;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells
Authors: C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto
Cell Rep, 2020-09-08;32(10):108105.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Western Blot -
The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo
Authors: YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao
Oxid Med Cell Longev, 2020-06-05;2020(0):1404915.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus
Authors: ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu
Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):.
Species: Virus - Henipavirus
Sample Types: Recombinant Protein
Applications: Coprecipitation Assay -
Fusogenicity of the Ghana Virus (Henipavirus: Ghanaian bat henipavirus) Fusion Protein is Controlled by the Cytoplasmic Domain of the Attachment Glycoprotein
Authors: K Voigt, M Hoffmann, JF Drexler, MA Müller, C Drosten, G Herrler, N Krüger
Viruses, 2019-08-29;11(9):.
Species: Virus - Ghana
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart
Authors: D Herrero, S Cañón, G Albericio, RM Carmona, S Aguilar, S Mañes, A Bernad
Redox Biol, 2019-03-04;22(0):101156.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Flow Cytometry -
Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling
Authors: JM Baek, YH Cheon, SC Kwak, HY Jun, KH Yoon, MS Lee, JY Kim
Exp. Mol. Med., 2018-04-27;50(4):50.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF
Authors: E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi
EMBO Rep., 2018-04-11;0(0):.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Cell Culture, In Vivo -
Intersectin 1 is a component of the Reelin pathway to regulate neuronal migration and synaptic plasticity in the hippocampus
Authors: B Jakob, G Kochlamaza, M Jäpel, A Gauhar, HH Bock, T Maritzen, V Haucke
Proc. Natl. Acad. Sci. U.S.A., 2017-05-08;114(21):5533-5538.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model
Mol Psychiatry, 2016-09-20;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase
Authors: M Wang, MJ Collins, TR Foster, H Bai, T Hashimoto, JM Santana, C Shu, A Dardik
J. Vasc. Surg., 2016-01-24;65(1):179-189.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-beta-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity.
Authors: Miyamoto T, Kim D, Knox J, Johnson E, Mucke L
J Biol Chem, 2015-11-20;291(4):1719-34.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation.
Authors: Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T
PLoS ONE, 2015-09-25;10(9):e0138835.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
EphB4 forward signalling regulates lymphatic valve development.
Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
Nat Commun, 2015-04-13;6(0):6625.
Species: Mouse
Sample Types: Antibody
Applications: Enzyme Assay -
GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z
J Neurosci, 2015-04-01;35(13):5409-21.
Species: Rat
Sample Types: Whole Tissue
Applications: Co-Immunoprecipitation -
Murine, but not human, ephrin-B2 can be efficiently cleaved by the serine protease kallikrein-4: implications for xenograft models of human prostate cancer.
Authors: Lisle J, Mertens-Walker I, Stephens C, Stansfield S, Clements J, Herington A, Stephenson S
Exp Cell Res, 2015-02-24;333(1):136-46.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Nipah virus attachment glycoprotein stalk C-terminal region links receptor binding to fusion triggering.
Authors: Liu Q, Bradel-Tretheway B, Monreal A, Saludes J, Lu X, Nicola A, Aguilar H
J Virol, 2014-11-26;89(3):1838-50.
Species: Virus
Sample Types: Whole Cells
Applications: Bioassay -
Biomaterial microenvironments to support the generation of new neurons in the adult brain.
Authors: Conway A, Schaffer D
Stem Cells, 2014-05-01;32(5):1220-9.
Applications: Bioassay -
The composition of EphB2 clusters determines the strength in the cellular repulsion response.
Authors: Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R
J Cell Biol, 2014-01-27;204(3):409-22.
Species: Human, Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Bioassay -
Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development.
Authors: Soskis, Michael, Ho, Hsin-Yi, Bloodgood, Brenda L, Robichaux, Michael, Malik, Athar N, Ataman, Bulent, Rubin, Alex A, Zieg, Janine, Zhang, Chao, Shokat, Kevan M, Sharma, Nikhil, Cowan, Christop, Greenberg, Michael
Nat Neurosci, 2012-11-11;15(12):1645-54.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
Authors: Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
Eur. J. Immunol., 2012-05-23;42(6):1562-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes.
Authors: Maddigan A, Truitt L, Arsenault R
J. Immunol., 2011-10-28;187(11):5983-94.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Ephrins negatively regulate cell proliferation in the epidermis and hair follicle.
Authors: Genander M, Holmberg J, Frisen J
Stem Cells, 2010-07-01;28(7):1196-205.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
p75NTR is an obligate signaling receptor required for cues that cause sympathetic neuron growth cone collapse.
Authors: Naska S, Lin DC, Miller FD
Mol. Cell. Neurosci., 2010-06-17;45(2):108-20.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis.
Authors: Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A
Nature, 2010-05-05;465(7297):487-91.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
Authors: Afshari FT, Kwok JC, Fawcett JW
J. Neurosci., 2010-03-24;30(12):4246-55.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro.
Authors: He S, Kumar SR, Zhou P, Krasnoperov V, Ryan SJ, Gill PS, Hinton DR
Invest. Ophthalmol. Vis. Sci., 2009-08-20;51(1):543-52.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth.
Authors: Pennisi A, Ling W, Li X, Khan S, Shaughnessy JD, Barlogie B, Yaccoby S
Blood, 2009-07-13;114(9):1803-12.
Species: Human
Sample Types: In Vivo
Applications: In Vivo -
EphrinB2 induces tyrosine phosphorylation of NR2B via Src-family kinases during inflammatory hyperalgesia.
Authors: Slack S, Battaglia A, Cibert-Goton V, Gavazzi I
Neuroscience, 2008-07-18;156(1):175-83.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Modulation of ephrinB2 leads to increased angiogenesis in ischemic myocardium and endothelial cell proliferation.
Authors: Mansson-Broberg A, Siddiqui AJ, Genander M, Grinnemo KH, Hao X, Andersson AB, Wardell E, Sylven C, Corbascio M
Biochem. Biophys. Res. Commun., 2008-06-20;373(3):355-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis.
Authors: Masuda J, Usui R, Maru Y
J. Biol. Chem., 2008-02-28;283(19):13148-55.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Plasticity of neuron-glial interactions mediated by astrocytic EphARs.
Authors: Nestor MW, Mok LP, Tulapurkar ME, Thompson SM
J. Neurosci., 2007-11-21;27(47):12817-28.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Single amino acid changes in the Nipah and Hendra virus attachment glycoproteins distinguish ephrinB2 from ephrinB3 usage.
Authors: Negrete OA, Chu D, Aguilar HC, Lee B
J. Virol., 2007-07-25;81(19):10804-14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth.
Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM
Biochemistry, 2007-01-24;46(7):1799-810.
Species: Human
Sample Types: Peptide
Applications: Binding Assay -
EphB4 provides survival advantage to squamous cell carcinoma of the head and neck.
Authors: Masood R, Kumar SR, Sinha UK, Crowe DL, Krasnoperov V, Reddy RK, Zozulya S, Singh J, Xia G, Broek D, Schonthal AH, Gill PS
Int. J. Cancer, 2006-09-15;119(6):1236-48.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
J. Biol. Chem., 2006-08-31;281(43):32574-86.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Immunoprecipitation -
EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.
Authors: Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J
Cell, 2006-06-16;125(6):1151-63.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
EphrinB3 regulates cell proliferation and survival in adult neurogenesis.
Authors: Ricard J, Salinas J, Garcia L, Liebl DJ
Mol. Cell. Neurosci., 2006-02-17;31(4):713-22.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Flow regulates arterial-venous differentiation in the chick embryo yolk sac.
Authors: le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, Breant C, Fleury V, Eichmann A
Development, 2003-12-17;131(2):361-75.
Species: Avian - Quail, Chicken
Sample Types: In Vivo, Whole Tissue
Applications: IHC, In Vivo -
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
J. Cell. Sci., 2003-05-06;116(0):2461-70.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: Bioassay, ICC, IHC -
Ephrin stimulation modulates T cell chemotaxis.
Authors: Sharfe N, Freywald A, Toro A, Dadi H, Roifman C
Eur. J. Immunol., 2002-12-01;32(12):3745-55.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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