Recombinant Mouse Epiregulin Protein Summary
Product Specifications
Val56-Leu101, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1068-EP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1068-EP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Epiregulin
Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a transmembrane precursor of 162 amino acid in length, with the mature soluble form comprising residues 56 - 101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6).
- Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
- Toyoda, H. et al. (1995) FEBS Lett. 377:403.
- Komurasaki, T. et al. (1997) Oncogene 15:2841.
- Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
- Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
- Das, S.K. et al. (1997) Dev. Biol. 190:178.
Citations for Recombinant Mouse Epiregulin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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CAPA-IVM improves the cytoplasmic quality of in vitro-matured oocytes from unstimulated mice
Authors: Gong, X;Shen, L;Zhang, H;Ai, J;Gilchrist, RB;Zhao, Y;
Theriogenology
Species: Mouse
Sample Types: Whole Cell
Applications: In vitro -
Tellu: an object detector algorithm for automatic classification of intestinal organoids
Authors: E Domènech-M, A Brandt, TT Lemmetyine, L Wartiovaar, TP Mäkelä, S Ollila
Disease Models & Mechanisms, 2023-03-13;0(0):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay -
Non-enzymatic role of SOD1 in intestinal stem cell growth
Authors: YC Wang, XX Leng, CB Zhou, SY Lu, CK Tsang, J Xu, MM Zhang, HM Chen, JY Fang
Cell Death & Disease, 2022-10-20;13(10):882.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay -
Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation
Authors: F Westendorp, ON Karpus, PJ Koelink, JLM Vermeulen, S Meisner, J Koster, NVJA Büller, ME Wildenberg, V Muncan, GR van den Br
Oncogene, 2021-01-21;0(0):.
Species: Transgenic Mouse
Sample Types: Organoid
Applications: Bioassay -
Epiregulin and EGFR interactions are involved in pain processing
Authors: LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko
J. Clin. Invest., 2017-08-07;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase is required for rapid resumption of oocyte meiosis in response to luteinizing hormone.
Authors: Shuhaibar L, Egbert J, Edmund A, Uliasz T, Dickey D, Yee S, Potter L, Jaffe L
Dev Biol, 2015-10-30;409(1):194-201.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
J Immunol, 2015-01-02;194(3):1039-46.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The aryl hydrocarbon receptor directly regulates expression of the potent mitogen epiregulin.
Authors: Patel RD, Kim DJ, Peters JM
Toxicol. Sci., 2005-09-28;89(1):75-82.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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