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Recombinant Mouse FGF-10 Protein

Catalog #: 6224-FG
30 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
6224-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
6224-FG-025
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Citations (30)
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Recombinant Mouse FGF-10 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-120 ng/mL
Source
E. coli-derived mouse FGF-10 protein
Ser62-Thr209
Accession #
NP_032028.1
N-terminal Sequence
Analysis
Ser62
Predicted Molecular Mass
17.1 kDa
SDS-PAGE
17 kDa, reducing conditions

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6224-FG (with carrier)

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6224-FG/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6224-FG

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6224-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-10

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature mouse FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). C-terminal to this region (aa 62 ‑ 209), it shares  94% and 100% amino acid sequence identity with human and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (2, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (11 - 14). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 9, 12 ‑ 14). The expression and function of FGF‑10 are negatively regulated by Shh and BMP-4 in the developing lung (2, 8). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF-10 biology (7, 9, 13, 14). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (15).

References
  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Bellusci, S. et al. (1997) Development 124:4867.
  3. Tagashira, S. et al. (1997) Gene 197:399.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Weaver, M. et al. (2000) Development 127:2695.
  9. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  10. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  11. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  12. Mailleux, A.A. et al. (2002) Development 129:53.
  13. Makarenkova, H.P. et al. (2000) Development 127:2563.
  14. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  15. Nomura, S. et al. (2008) Br. J. Cancer 99:305.
Long Name
Fibroblast Growth Factor 10
Entrez Gene IDs
2255 (Human); 14165 (Mouse); 25443 (Rat)
Alternate Names
FGF10; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; KGF2; KGF-2; produced by fibroblasts of urinary bladder lamina propria

Citations for Recombinant Mouse FGF-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Specific 3-O-sulfated heparan sulfate domains regulate salivary gland basement membrane metabolism and epithelial differentiation
    Authors: Patel, VN;Aure, MH;Choi, SH;Ball, JR;Lane, ED;Wang, Z;Xu, Y;Zheng, C;Liu, X;Martin, D;Pailin, JY;Prochazkova, M;Kulkarni, AB;van Kuppevelt, TH;Ambudkar, IS;Liu, J;Hoffman, MP;
    Nature communications
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. FGFR2 is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation
    Authors: Aure, MH;Symonds, JM;Villapudua, CU;Dodge, JT;Werner, S;Knosp, WM;Hoffman, MP;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling
    Authors: DG Brownfield, AD de Arce, E Ghelfi, A Gillich, TJ Desai, MA Krasnow
    Nature Communications, 2022-11-21;13(1):7137.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes
    Authors: H Kim, BW Song, SJ Park, SW Choi, H Moon, KC Hwang, SW Kang, SH Moon, Y Yang, IC Kwon, SH Kim
    Science Advances, 2022-02-25;8(8):eabj6621.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Induction of gammadeltaT cells from HSC-enriched BMCs co-cultured with iPSC-derived thymic epithelial cells
    Authors: N Hosaka, S Kanda, T Shimono, T Nishiyama
    Journal of Cellular and Molecular Medicine, 2021-10-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas
    Authors: H Tomita, K Tanaka, A Hirata, H Okada, H Imai, Y Shirakami, K Ohnishi, S Sugie, H Aoki, Y Hatano, K Noguchi, T Kanayama, A Niwa, N Suzui, T Miyazaki, T Tanaka, H Akiyama, M Shimizu, K Yoshida, A Hara
    Cell Reports, 2021-02-23;34(8):108772.
    Species: Mouse
    Sample Types: Reference Standard
  7. Epithelial expression of Gata4 and Sox2 regulates specification of the squamous-columnar junction via MAPK/ERK signaling in mice
    Authors: N Sankoda, W Tanabe, A Tanaka, H Shibata, K Woltjen, T Chiba, H Haga, Y Sakai, M Mandai, T Yamamoto, Y Yamada, S Uemoto, Y Kawaguchi
    Nature Communications, 2021-01-25;12(1):560.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction
    Authors: H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata
    Cell Stem Cell, 2020-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Drug-Induced Na�ve iPS Cells Exhibit Better Performance than Primed iPS Cells with Respect to the Ability to Differentiate into Pancreatic &beta-Cell Lineage
    Authors: Y Kiyokawa, M Sato, H Noguchi, E Inada, Y Iwase, N Kubota, T Sawami, M Terunuma, T Maeda, H Hayasaki, I Saitoh
    Journal of Clinical Medicine, 2020-09-02;9(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors
    Authors: KA Lloyd, BN Parsons, MD Burkitt, AR Moore, S Papoutsopo, M Boyce, CA Duckworth, K Exarchou, N Howes, L Rainbow, Y Fang, C Oxvig, S Dodd, A Varro, N Hall, DM Pritchard
    Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  11. Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor
    Authors: T Momozane, E Fukui, S Funaki, M Fujii, Y Kinehara, E Ito, S Miyagawa, Y Ohno, Y Sawa, M Okumura, Y Shintani
    Stem Cells Int, 2019-12-27;2019(0):1271682.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals
    Authors: T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim
    Nat Commun, 2019-10-11;10(1):4647.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Exploring the Molecular Crosstalk between Pancreatic Bud and Mesenchyme in Embryogenesis: Novel Signals Involved
    Authors: I Guerriero, MT De Angelis, F D'Angelo, R Leveque, E Savignano, L Roberto, V Lucci, P Mazzone, S Laurino, G Storto, A Nardelli, A Sgambato, M Ceccarelli, M De Felice, E Amendola, G Falco
    Int J Mol Sci, 2019-10-03;20(19):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  14. Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes
    Authors: S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme
    Sci Rep, 2019-06-11;9(1):8470.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA
    Authors: C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi
    Sci Rep, 2018-08-17;8(1):12341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Barhl1 is required for the differentiation of inner ear hair cell-like cells from mouse embryonic stem cells
    Authors: C Zhong, Z Chen, X Luo, C Wang, H Jiang, J Shao, M Guan, L Huang, X Huang, J Wang
    Int. J. Biochem. Cell Biol., 2018-02-06;96(0):79-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  17. Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells
    Authors: MA Scavuzzo, D Yang, M Borowiak
    Sci Rep, 2017-09-07;7(1):10810.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  18. Fgf10-Sox9 are essential for establishment of distal progenitor cells during salivary gland development
    Authors: L Chatzeli, M Gaete, AS Tucker
    Development, 2017-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  19. Stepwise reprogramming of liver cells to a pancreas progenitor state by the transcriptional regulator Tgif2
    Authors: N Cerdá-Este, H Naumann, S Ruzittu, N Mah, IM Pongrac, C Cozzitorto, A Hommel, MA Andrade-Na, E Bonifacio, FM Spagnoli
    Nat Commun, 2017-02-13;8(0):14127.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Wnt/?-catenin promotes gastric fundus specification in mice and humans
    Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
    Nature, 2017-01-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  21. Tissue-Specific Stem Cells Obtained by Reprogramming of Non-Obese Diabetic (NOD) Mouse-Derived Pancreatic Cells Confer Insulin Production in Response to Glucose
    PLoS ONE, 2016-09-23;11(9):e0163580.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair
    Authors: Jennifer Quantius
    PLoS Pathog, 2016-06-20;12(6):e1005544.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  23. Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage
    Authors: Marie-Isabelle Garcia
    Development, 2016-03-17;143(9):1452-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline.
    Authors: Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T
    PLoS ONE, 2015-06-15;10(6):e0130147.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  25. Mesenchymal Wnt/beta-Catenin Signaling Controls Epithelial Stem Cell Homeostasis in Teeth by Inhibiting the Antiapoptotic Effect of Fgf10.
    Authors: Yang Z, Balic A, Michon F, Juuri E, Thesleff I
    Stem Cells, 2015-05-01;33(5):1670-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  26. Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection.
    Authors: Noguchi, H, Saitoh, I, Tsugata, T, Kataoka, H, Watanabe, M, Noguchi, Y
    Cell Death Differ, 2014-09-05;22(1):145-55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Role of heparan sulfate proteoglycans in optic disc and stalk morphogenesis.
    Authors: Cai Z, Grobe K, Zhang X
    Dev Dyn, 2014-05-06;243(10):1310-6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  28. Mice with Tak1 deficiency in neural crest lineage exhibit cleft palate associated with abnormal tongue development.
    Authors: Song Z, Liu C, Iwata J, Gu S, Suzuki A, Sun C, He W, Shu R, Li L, Chai Y, Chen Y
    J Biol Chem, 2013-03-04;288(15):10440-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  29. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012-10-07;491(7426):765-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  30. miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis.
    Authors: Rebustini IT, Hayashi T, Reynolds AD
    Development, 2011-11-24;139(1):191-202.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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