Recombinant Mouse FGFR1 Fc Chimera Protein, CF

Catalog #: 10135-FR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10135-FR-050

Recombinant Mouse FGFR1 Fc Chimera Protein Inhibition Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse FGFR1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 15-90 ng/mL

Source

Mouse myeloma cell line, NS0-derived mouse FGFR1 protein

Mouse FGF R1 (Arg22-Ile376) Accession # P16092-6	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

P16092-6

N-terminal Sequence
Analysis

Arg22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

66 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Product Datasheets

10135-FR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10135-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Inhibition Activity

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Recombinant Mouse FGF R1 Fc Chimera (Catalog # 10135-FR) inhibits Recombinant Mouse FGF acidic (Catalog # 4686-FA) induced proliferation of NR6R-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 15-90 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse FGF R1 Fc Chimer (Catalog # 10135-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively.

Reconstitution Calculator

Background: FGFR1

Fibroblast growth factor receptor 1 (FGF R1) is a member of a tyrosine kinase family that acts as cell-surface receptors for fibroblast growth factors (FGFs). The FGF R family consists of four members that differ in their ligand affinities and play essential roles in the regulation of embryonic development, cell proliferation, differentiation, and migration (1, 2). Mature mouse FGF R1 consists of a 355 amino acid (aa) extracellular domain (ECD) composed of three immunoglobulin-like domains, a single transmembrane domain and a cytoplasmic tyrosine kinase domain (1). The ECD of mouse FGF R1 shares 98% and 99% aa identity with the human and rat ECD, respectively. FGF R1 binds both acidic and basic fibroblast growth factors and is involved in limb induction (2, 3). Alternative splice variants of this gene have been reported. This product is the longest variant, isoform 1 (1, 4). FGF Rs play essential roles in the regulation of embryonic development, cell proliferation, differentiation and migration (5). Upon FGF and heparan sulfate binding, the FGF Rs form homodimers and the kinase domains are activated (6, 7). Alterations in the binding regulates FGF bioactivity (8).

References

Beer, H.-D. et al. (2000) J. Biol. Chem. 275:16091.
Ornitz, D.M. et al. (1992) Mol. Cell. Bio. 12:240.
Cuiling, Li. et al. (2005) Dev. 132:4755.
Kouhara, H. et al. (1991) Biochem. Biophys. Res. Commun. 176:31.
Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116.
Pantoliano, M.W. et al. (1994) Biochemistry 33:10229.
Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.

Long Name

Fibroblast Growth Factor Receptor 1

Entrez Gene IDs

2260 (Human); 14182 (Mouse)

Alternate Names

basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331 antigen; CD331; CEK; EC 2.7.10; EC 2.7.10.1; FGF R1; FGFBR; FGFR1; FGFR-1; fibroblast growth factor receptor 1; FLGH3; FLJ99988; Flt-2; FLT2H4; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; H2; H5; HBGFR; heparin-binding growth factor receptor; hydroxyaryl-protein kinase; KAL 2; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2