Recombinant Mouse Frizzled-1 Fc Chimera Protein, CF

Catalog #: 1120-FZ
2 Citations Datasheet / COA / SDS

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1120-FZ-050

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Frizzled-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind biotinylated Wnt-3a in a functional ELISA with an estimated K_D < 10 nM.

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-1 protein

Mouse Frizzled-1 (Gln72 - His248) with substitution Met122Ile Accession # O70421	IEGRMD	Human IgG1 (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

O70421

N-terminal Sequence
Analysis

No results obtained: Gln 72 predicted & Ala74

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

55-62 kDa, reducing conditions

Product Datasheets

1120-FZ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1120-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Frizzled-1

Frizzled-1 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1 - 4). Frizzled proteins are thought to be G-protein-coupled (1, 5). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta ‑catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzled-1 can also act as a Wnt antagonist, for example, when sequestered from LRP-5/6 by LRP-1 (6, 7). Mouse Frizzled-1 cDNA encodes 642 aa including a 68 aa signal peptide, an extracellular cysteine-rich domain (CRD, aa 106 - 225) that binds Wnts and is highly conserved among Frizzleds, a linker region, the 7TM region, and the C-terminal cytoplasmic domain (2, 3). Most Frizzleds, including Frizzled-1, contain a C‑terminal PDZ binding motif (1). Within aa 72 - 248, mouse Frizzled-1 shares 94%, 97%, 97% and 93% aa identity with human, rat, bovine and canine Frizzled‑1, respectively. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 8). Frizzled-1 shares high aa identity (~76%) with Frizzled-2 and -7, but may differ in expression and Wnt selectivity (4, 9). Specifically, Wnt-3a interaction with rat Frizzled-1, but not Frizzled-2, blocks toxicity of the Alzheimers A beta peptide on PC12 cells (9). In bone, Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 on mesenchymal cells (7, 10). Frizzled-1 is also one of the purported Wnt10b receptors whose signaling inhibits adipocyte differentiation (11). It is widely expressed, with mRNA detection in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (2, 3).

References

Schulte, G. and B. Vitezslav (2007) Trends Pharmacol. Sci. 28:518.
Xu, L.et al. (2001) Matrix Biol. 20:147.
Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
Gazit, A. et al. (1999) Oncogene 18:5959.
Liu, T. et al. (1999) J. Biol. Chem. 274:33539.
Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
Kaykas, A. et al. (2004) Nat. Cell Biol. 6:52.
Chacon, M.A. et al. (2008) J. Cell. Physiol. 217:215.
Yerges, L.M. et al. (2009) J. Bone Miner. Res. 24:87.
Bennett, C.N. et al. (2002) J. Biol. Chem. 277:30998.

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Alternate Names

DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor

Related Research Areas

Citations for Recombinant Mouse Frizzled-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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