Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF

Catalog #: 9247-FZ Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9247-FZ-050

Recombinant Mouse Frizzled-10 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED₅₀ of 10-60 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-10 protein

Mouse Frizzled-10 (Ile22-Phe166) Accession # Q8BKG4	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q8BKG4

N-terminal Sequence
Analysis

Ile22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

44 kDa

SDS-PAGE

53-61 kDa, reducing conditions

Product Datasheets

9247-FZ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9247-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Frizzled-10 Fc Chimera (Catalog # 9247-FZ) binds to Biotinylated Recombinant Mouse Wnt-3a in an ELISA Binding Assay. When Biotinylated Recombinant Mouse Wnt-3a is coated on a streptavidin coated ELISA plate, Recombinant Mouse Frizzled-10 Fc Chimera binds with an ED₅₀ = 10-60 ng/mL.

Reconstitution Calculator

Background: Frizzled-10

Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the frizzled family of Wnt receptors (1, 2). The N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3-5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). Within the cysteine-rich domain, human Frizzled-10 shares 94% and 99% aa sequence identity with human and rat Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6-12). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (13, 14). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 and transduces Wnt-1, -7a, -7b, and -8 signals, resulting in the stabilization of cytoplasmic beta-Catenin (11, 12, 14). It is important for the differentiation of sensory neurons (12). Frizzled-10 is also up-regulated in some cancers and transformed cell lines (4, 15). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (16).

References