Recombinant Mouse Frizzled-4 Fc Chimera Protein, CF Summary
Product Specifications
| Frizzled-4 (Phe37-Glu180) Accession # Q8BLL2 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
194-FZ
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Frizzled-4
Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120 amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89% and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).
- Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
- Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
- Xu, Q. et al. (2004) Cell 116:883.
- Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
- Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
- Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
- Wang, Y. et al. (2001) J. Neurosci. 21:4761.
- Robitaille, J. et al. (2002) Nat. Genet. 32:326.
- Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.
Citations for Recombinant Mouse Frizzled-4 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo
Authors: Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia
The Journal of Biological Chemistry, 2022-05-04;0(0):102010.
Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture -
Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling.
Authors: Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST
J. Biol. Chem., 2008-01-30;283(14):9359-68.
Species: Human
Sample Types: Recombinant Protein
Applications: Phage Display -
Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
Authors: Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
Mol. Biol. Cell, 2006-10-11;17(12):5163-72.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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