Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF

Catalog #: 2440-FZ
1 Citation Datasheet / COA / SDS

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2440-FZ-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a funtional ELISA with an estimated K_d<10 nM.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Frizzled-9 protein

Mouse Frizzled-9 (Leu24-Asp186) Accession # Q9R216	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9R216

N-terminal Sequence
Analysis

Leu24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

58-66 kDa, reducing conditions

Product Datasheets

2440-FZ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2440-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Frizzled-9

Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mature mouse Frizzled-9 consists of a 207 amino acid (aa) N-terminal extracellular domain (ECD), the 7TM region, and a 62 aa C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region that binds Wnts and is highly conserved among Frizzleds. Within aa 23-186 of the N-terminal ECD, mouse Frizzled-9 shares 97% and 100% aa identity with human and rat Frizzled-9, respectively. The human Frizzled-9 gene maps to a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the central nervous system and myotomes during development, and it persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, differentiating osteoblasts, and developing myotubes of skeletal muscle (4-7). Mice lacking Frizzled-9 exhibit defects in learning, B lymphocyte development, and bone mineralization (4-6, 8). Frizzled-9 can signal through both canonical and non-canonical pathways (6, 9). Its binding to Wnt-7a promotes the activation of PPAR gamma, expression of Cadherin and Sprouty, and reversal of non-small cell lung cancer cell transformation (10-12).

References

Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171:1195.
Wang, Y.K. et al. (1997) Hum. Mol. Genet. 6:465.
Wang, Y.K. et al. (1999) Genomics 57:235.
Zhao, C. et al. (2005) Development 132:2917.
Ranheim, E.A. et al. (2005) Blood 105:2487.
Albers, J. et al. (2011) J. Cell Biol. 192:1057.
Aviles, E.C. et al. (2014) Front. Cell. Neurosci. 8:110.
Heilmann, A. et al. (2013) PLoS One 8:e84232.
Karasawa, T. et al. (2002) J. Biol. Chem. 277:37479.
Winn, R.A. et al. (2005) J. Biol. Chem. 280:19625.
Winn, R.A. et al. (2006) J. Biol. Chem. 281:26943.
Tennis, M.A. et al. (2010) Mol. Cancer Res. 8:833.

Entrez Gene IDs

8326 (Human); 14371 (Mouse)

Alternate Names

CD349 antigen; CD349; frizzled (Drosophila) homolog 9; frizzled homolog 9 (Drosophila); Frizzled9; Frizzled-9; fz-9; FZD3fzE6; FZD9; FzE6; hFz9

Related Research Areas

Citation for Recombinant Mouse Frizzled-9 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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