Recombinant Mouse Gas6 Protein Summary
Product Specifications
Asp115-Pro673, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
986-GS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
986-GS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Gas6
Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium.
Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.
- Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
- Goruppi, S. et al. (1996) Oncogene 12:471.
- Crosier, K and P. Crosier (1997) Pathology 29:131.
- Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
- Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.
Citations for Recombinant Mouse Gas6 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver
Authors: Tsugawa, H;Ohki, T;Tsubaki, S;Tanaka, R;Matsuzaki, J;Suzuki, H;Hozumi, K;
PLoS pathogens
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Gas6 induces inflammation and reduces plaque burden but worsens behavior in a sex-dependent manner in the APP/PS1 model of Alzheimer's disease
Authors: LD Owlett, B Karaahmet, L Le, EK Belcher, D Dionisio-S, JA Olschowka, MR Elliott, MK O'Banion
Journal of Neuroinflammation, 2022-02-07;19(1):38.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury
Authors: H Zhou, L Hu, J Li, W Ruan, Y Cao, J Zhuang, H Xu, Y Peng, Z Zhang, C Xu, Q Yu, Y Li, Z Dou, J Hu, X Wu, X Yu, C Gu, S Cao, F Yan, G Chen
Journal of Neuroinflammation, 2021-07-07;18(1):154.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Respiratory syncytial virus infection exacerbates pneumococcal pneumonia via Gas6/Axl-mediated macrophage polarization
Authors: T Shibata, A Makino, R Ogata, S Nakamura, T Ito, K Nagata, Y Terauchi, T Oishi, M Fujieda, Y Takahashi, M Ato
J. Clin. Invest., 2020-06-01;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Novel AXL-specific inhibitor ameliorates kidney dysfunction through the inhibition of epithelial-to-mesenchymal transition of renal tubular cells
Authors: A Kurata, Y Tachibana, T Takahashi, N Horiba
PLoS ONE, 2020-04-23;15(4):e0232055.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
TAM receptors attenuate murine NK-cell responses via E3 ubiquitin ligase Cbl-b
Authors: LM Chirino, S Kumar, M Okumura, DE Sterner, M Mattern, TR Butt, T Kambayashi
Eur. J. Immunol., 2019-09-30;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Gas6/Axl signaling attenuates alveolar inflammation in ischemia-reperfusion-induced acute lung injury by up-regulating SOCS3-mediated pathway
Authors: CK Peng, CP Wu, JY Lin, SC Peng, CH Lee, KL Huang, CH Shen
PLoS ONE, 2019-07-18;14(7):e0219788.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Cell Culture -
Gas6 Prevents Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells via Production of PGE2, PGD2 and Their Receptors
Authors: J Jung, YJ Lee, YH Choi, EM Park, HS Kim, JL Kang
Cells, 2019-06-26;8(7):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Gas6 Attenuates Sepsis-Induced Tight Junction Injury and Vascular Endothelial Hyperpermeability via the Axl/NF-kappaB Signaling Pathway
Authors: J Ni, M Lin, Y Jin, J Li, Y Guo, J Zhou, G Hong, G Zhao, Z Lu
Front Pharmacol, 2019-06-13;10(0):662.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
MERTK mediated novel site Akt phosphorylation alleviates SAV1 suppression
Authors: Y Jiang, Y Zhang, JY Leung, C Fan, KI Popov, S Su, J Qian, X Wang, A Holtzhause, E Ubil, Y Xiang, I Davis, NV Dokholyan, G Wu, CM Perou, WY Kim, HS Earp, P Liu
Nat Commun, 2019-04-03;10(1):1515.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Stromal Gas6 promotes the progression of premalignant mammary cells
Authors: AM Gomes, EC Carron, KL Mills, AM Dow, Z Gray, CR Fecca, MA Lakey, P Carmeliet, F Kittrell, D Medina, HL Machado
Oncogene, 2018-12-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
Neuron, 2018-03-07;97(5):1023-1031.e7.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
Neuron, 2017-05-03;94(3):500-516.e9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.
Authors: Lee Y, Park H, Woo S, Park E, Kang J
J Pharmacol Exp Ther, 2014-06-17;350(3):563-77.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
TAM receptors affect adult brain neurogenesis by negative regulation of microglial cell activation.
Authors: Ji R, Tian S, Lu H, Lu Q, Zheng Y, Wang X, Ding J, Li Q, Lu Q
J Immunol, 2013-11-15;191(12):6165-77.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
Authors: Deng T, Zhang Y, Chen Q, Yan K, Han D
Immunology, 2012-01-01;135(1):40-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
An anti-Axl monoclonal antibody attenuates xenograft tumor growth and enhances the effect of multiple anticancer therapies.
Authors: Ye X, Li Y, Stawicki S
Oncogene, 2010-07-05;29(38):5254-64.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Binding Assay -
Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
J. Immunol., 2009-08-05;183(5):3004-13.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice.
Authors: Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A
J. Hepatol., 2009-04-22;51(1):55-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
Authors: Uehara H, Shacter E
J. Immunol., 2008-02-15;180(4):2522-30.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Role of Gas6 in erythropoiesis and anemia in mice.
Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
J. Clin. Invest., 2008-02-01;118(2):583-96.
Applications: ELISA (Standard) -
TAM receptors are pleiotropic inhibitors of the innate immune response.
Authors: Rothlin CV, Ghosh S, Zuniga EI, Oldstone MB, Lemke G
Cell, 2007-12-14;131(6):1124-36.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
Authors: Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
Blood, 2006-10-17;109(3):1026-33.
Species: Mouse
Sample Types: Recombinant Protein, Serum
Applications: Bioassay, Immunoprecipitation
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