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Recombinant Mouse GDF-6/BMP-13 Protein

Catalog #: 855-G6
4 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
855-G6-050/CF

With Carrier

Catalog # Availability Size / Price Qty
855-G6-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (4)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse GDF-6/BMP-13 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is typically 0.85-5 µg/mL.
Source
E. coli-derived mouse GDF-6/BMP-13 protein
Thr335-Arg454
Accession #
P43028
N-terminal Sequence
Analysis
Thr335
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13.7 kDa (monomer)

Product Datasheets

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855-G6 (with carrier)

Product Datasheet
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SDS

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855-G6/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

855-G6

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 300 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

855-G6/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 300 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-6/BMP-13

Growth Differentiation Factor 6 (GDF-6), also known as bone morphogenetic protein 13 (BMP-13) or cartilage-derived morphogenetic protein 2 (CDMP-2), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. The mature GDF-6 with 120 amino acids is a homodimeric protein containing the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-6 is highly conserved across species. At the amino acid sequence level, human and mouse GDF‑6 are 99% identical. It has been reported that GDF-6 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-6 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Inada, M. et al. (1996) BBRC, 222:317.
  6. Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
Long Name
Growth Differentiation Factor 6
Entrez Gene IDs
392255 (Human); 242316 (Mouse)
Alternate Names
BMP13; BMP-13; CDMP2; GDF16; GDF6; GDF-6; growth differentiation factor 6; Growth/differentiation factor 16; growth/differentiation factor 6; KFS; KFS1; KFSL; MCOP4; MCOPCB6; MGC158100; MGC158101; SCDO4; SGM1

Citations for Recombinant Mouse GDF-6/BMP-13 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Fibroblast growth factor 8b (FGF-8b) enhances myogenesis and inhibits adipogenesis in rotator cuff muscle cell populations in vitro
    Authors: Otsuka, T;Kan, HM;Mengsteab, PY;Tyson, B;Laurencin, CT;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Control of mesenchymal cell fate via application of FGF-8b in vitro
    Authors: T Otsuka, PY Mengsteab, CT Laurencin
    Stem Cell Research, 2021-01-07;51(0):102155.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012-08-31;189(7):3508-20.
    Species: Mouse
    Sample Types: N/A, Whole Cells
    Applications: Bioassay, ELISA (Standard)
  4. Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein.
    Authors: Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R
    Int. J. Dev. Biol., 2003-02-01;47(1):15-29.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo

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