Recombinant Mouse GDF-8 Propeptide Protein Summary
Product Specifications
Asn25-Ser265, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1539-PG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1539-PG/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: GDF-8/Myostatin
Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1-3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF‑8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF‑8 propeptide functions as an inhibitor of mature GDF‑8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK‑4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).
- McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.
- McPherron, A.C. et al. (1997) Nature 387:83.
- Zimmers, T.A. et al. (2002) Science 296:1486.
- Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.
- McFarlane, C. et al. (2005) Dev. Biol. 283:58.
- Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.
- Thies, R.S. et al. (2001) Growth Factors 18:251.
- Amthor, H. et al. (2004) Dev. Biol. 270:19.
- Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.
- Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
- Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.
- Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.
- Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.
- Guo, W. et al. (2008) J. Biol. Chem. 283:9136.
- Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.
- Guo, T. et al. (2009) PloS ONE 4:e4937.
Citations for Recombinant Mouse GDF-8 Propeptide Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli
Authors: SB Lee, SK Park, YS Kim
PLoS ONE, 2017-04-03;12(4):e0174956.
Species: Human, Mouse
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Enzyme Assay -
A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
J. Mol. Biol., 2008-07-02;381(4):1025-39.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Developmet, Surface Plasmon Resonance -
Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
J. Biol. Chem., 2008-03-13;283(20):13874-88.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay, Surface Plasmon Resonance -
Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition.
Authors: Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, Tsuchida K, Noji S, Sunada Y
J. Clin. Invest., 2006-10-12;116(11):2924-34.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Control
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