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Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

Catalog #: 9844-GR Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9844-GR-050
Recombinant Mouse GFR alpha-like Fc Chimera Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse GFR alpha ‑like Fc Chimera is coated at 0.1 μg/mL, 100 μL/well, it binds to Biotinylated Recombinant Mouse GDF-15 with an ED50 of 0.3-1.8 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse GFR alpha-like protein
Mouse GFR alpha-like
(Gln20-Gly349)
Accession # Q6SJE0-1		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
Q6SJE0-1
N-terminal Sequence
Analysis
No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Thr21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
64 kDa
SDS-PAGE
66-85 kDa, reducing conditions

Product Datasheets

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9844-GR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9844-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse GFR alpha-like Fc Chimera Protein Bioactivity View Larger

When Recombinant Mouse GFR alpha ‑like Fc Chimera (Catalog # 9844-GR) is immobilized at 0.1 μg/mL, 100 μL/well, It binds to Biotinylated Recombinant Mouse GDF-15 with an ED50 of 0.3-1.8 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-like

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

References

  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Yang, L. et al. (2017) Nature Medicine 23:1158.
  3. Mullican, S. et al. (2017) Nature medicine 23:1150.

  4. Emmerson, P. et al. (2017) Nature Medicine 23:1215.

Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
bA360D14.1; C6orf144; GDNF family receptor alpha-like; GFR alpha like; GFR alpha-like; GFRAL; GRAL; IVFI9356; UNQ9356

FAQs

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