Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF

Name: Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 524-GR
Rating: 4.5 (2 reviews)

Catalog #: 524-GR 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
524-GR-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse GITR/TNFRSF18 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rmGITR/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmGITR Ligand with a linear range of 0.8-50 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse GITR/TNFRSF18 protein

Mouse GITR (Ser22-His153) Accession # Q8C4K3	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q8C4K3

N-terminal Sequence
Analysis

Ser22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

42 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Product Datasheets

524-GR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

524-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature mouse GITR consists of a 134 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (3). Within the ECD, mouse GITR shares 55% and 86% aa sequence identity with human and rat GITR, respectively. Alternative splicing generates additional isoforms with substituted cytoplasmic domains or with a substitution within the third TNFR repeat, transmembrane, and cytoplasmic regions (4). GITR is expressed on CD4⁺CD25⁺ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (3, 5, 6), and it is upregulated on antigen-activated conventional CD4⁺ and CD8⁺ T cells (3, 6-8). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4⁺ or CD8⁺ conventional T cells (7-10). It also induces the proliferation of Treg (9, 11) but inhibits the ability of Treg to suppress immune responses (5, 9, 12-14). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 12), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (11, 15). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (16).

References

Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
Ko, K. et al. (2005) J. Exp. Med. 202:885.
Ji, H. et al. (2004) J. Immunol. 172:5823.
Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Entrez Gene IDs

8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)

Alternate Names

Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18