Recombinant Mouse Glycoprotein V/CD42d Protein, CF

Catalog #: 6990-GP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
6990-GP-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Glycoprotein V/CD42d Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Glycoprotein V/CD42d is coated at 5 μg/mL, the concentration of Recombinant Human vWF-A2 (Catalog # 2764-WF) that produces 50% optimal binding response is found to be approximately 0.25-1.25 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Glycoprotein V/CD42d protein
Met1-Gly522 with a C-terminal 6-His tag

Accession #

NP_032174

N-terminal Sequence
Analysis

Gln17

Structure / Form

Dimer

Predicted Molecular Mass

57.5 kDa (monomer)

SDS-PAGE

70-90 kDa, reducing conditions

Product Datasheets

6990-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6990-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Glycoprotein V/CD42d

Platelet Glycoprotein V (GPV or GP5, designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI-V-IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard-Soulier syndrome, a rare bleeding disorder (1 - 3). The mouse GPV cDNA encodes 567 amino acids (aa), including a 16 aa signal sequence, a 506 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (24 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The mouse GPV ECD shares 70%, 87%, 68 and 67% aa identity with human, rat, equine and bovine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after L508, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6 - 8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11 ‑ 15).

References