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Recombinant Mouse gp130 Fc Chimera Protein, CF

Catalog #: 468-MG
10 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
468-MG-100
Recombinant Mouse gp130 Fc Chimera Protein Bioactivity
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse gp130 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED50 for this effect is 0.12-1.2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse gp130 protein
Mouse gp130
(Gln23-Glu617)
Accession # Q6PDI9		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
Q6PDI9
N-terminal Sequence
Analysis
No results obtained: Gln23 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
94 kDa (monomer)
SDS-PAGE
115 kDa, reducing conditions

Product Datasheets

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468-MG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

468-MG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse gp130 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG) inhibits IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED50 for this effect is 0.12-1.2 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References
  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

Citations for Recombinant Mouse gp130 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Administration of soluble gp130Fc disrupts M-1 macrophage polarization, dendritic cell activation, MDSC expansion and Th-17 induction during experimental cerebral malaria
    Authors: Mukherjee, S;Ghosh, S;Bawali, S;Chatterjee, R;Saha, A;Sengupta, A;Keswani, T;Sarkar, S;Ghosh, P;Chakraborty, S;Khamaru, P;Bhattacharyya, A;
    International immunopharmacology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. The fetal response to maternal inflammation is dependent upon maternal IL-6 in a murine model
    Authors: Bermick, J;Watson, S;Lueschow, S;McElroy, SJ;
    Cytokine
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/&beta1 integrin pathway
    Authors: S Yoshizaki, T Tamaru, M Hara, K Kijima, M Tanaka, DJ Konno, Y Matsumoto, Y Nakashima, S Okada
    Journal of Neuroinflammation, 2021-01-06;18(1):12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Diabetes Induced Alterations in Murine Vitreous Proteome Are Mitigated by IL-6 Trans-Signaling Inhibition
    Authors: R Robinson, H Youngblood, H Iyer, J Bloom, TJ Lee, L Chang, Z Lukowski, W Zhi, A Sharma, S Sharma
    Invest. Ophthalmol. Vis. Sci., 2020-09-01;61(11):2.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Interleukin-6 trans-signaling inhibition prevents oxidative stress in a mouse model of early diabetic retinopathy
    Authors: R Robinson, M Srinivasan, A Shanmugam, A Ward, V Ganapathy, J Bloom, A Sharma, S Sharma
    Redox Biol, 2020-05-13;34(0):101574.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  6. Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages
    Authors: G Trimova, K Yamagata, S Iwata, S Hirata, T Zhang, F Uemura, M Satoh, N Biln, S Nakayamada, WP Maksymowyc, Y Tanaka
    Arthritis Res. Ther., 2020-02-12;22(1):24.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Cell Culture
  7. Frontline Science: Proliferation of Ly6C(+) monocytes during urinary tract infections is regulated by IL-6 trans-signaling
    Authors: A Dixit, J Bottek, AL Beerlage, J Schuettpel, S Thiebes, A Brenzel, C Garbers, S Rose-John, HW Mittrücker, A Squire, DR Engel
    J. Leukoc. Biol., 2017-12-28;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression
    Authors: H Tsukamoto, K Fujieda, M Hirayama, T Ikeda, A Yuno, K Matsumura, D Fukuma, K Araki, H Mizuta, H Nakayama, S Senju, Y Nishimura
    Cancer Res, 2017-02-24;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Interleukin-6 induces S100A9 expression in colonic epithelial cells through STAT3 activation in experimental ulcerative colitis.
    Authors: Lee, Min Jeou, Lee, Jin-Ku, Choi, Ji Won, Lee, Chang-Se, Sim, Ji Hyun, Cho, Chung-Hy, Lee, Kwang-Ho, Cho, Ik-Hyun, Chung, Myung-He, Kim, Hang-Rae, Ye, Sang-Kyu
    PLoS ONE, 2012-09-04;7(9):e38801.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
    Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
    J. Immunol., 2010-01-18;184(4):2130-9.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo

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Recombinant Mouse gp130 Fc Chimera Protein, CF
By Anonymous on 01/08/2019
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Mouse gp130 Fc Chimera Protein, CF 468-MG