Home / HVEM/TNFRSF14 / Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

Catalog #: 2516-HV
1 Citation Datasheet / COA / SDS
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2516-HV-100
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse (rm) HVEM Fc Chimera is immobilized at 100 ng/ mL, 100 μL/well, the concentration of biotinylated rmBTLA Fc Chimera that produces 50% of the optimal binding response is found to be approximately 30-120 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse HVEM/TNFRSF14 protein
Mouse HVEM
(Gln39-Val207)
Accession # NP_849262		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
NP_849262
N-terminal Sequence
Analysis
Gln39
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45.6 kDa
SDS-PAGE
60-65 kDa, reducing conditions

Product Datasheets

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2516-HV

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2516-HV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature mouse HVEM consists of a 170 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 24 aa transmembrane segment, and a 45 aa cytoplasmic tail with a TRAF interaction domain (2). Within the ECD, mouse HVEM shares 55% and 89% aa sequence identity with human and rat HVEM, respectively. HVEM is highly expressed on naïve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (3-7). Its expression declines during effector T cell activation but is upregulated during Treg activation (3, 4). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (3, 8-11). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (7, 9) and contributes to Th1 inflammation and cardiac allograft rejection (12, 13). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (3, 6, 8, 9) and dampens intestinal inflammation (14). HVEM enhances the development of CD8+ T cell memory and Treg function (4, 5). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (15). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (Montgomery, 8, 10, 16).

References
  1. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
  2. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  3. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  4. Tao, R. et al. (2008) J. Immunol. 180:6649.
  5. Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
  6. de Trez, C. et al. (2008) J. Immunol. 180:238.
  7. Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
  8. Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
  9. Cai, G. et al. (2008) Nat. Immunol. 9:176.
  10. Mauri, D.N. et al. (1998) Immunity 8:21.
  11. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  12. Wang, J. et al. (2005) J. Immunol. 174:8173.
  13. Ye, Q. et al. (2002) J. Exp. Med. 195:795.
  14. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
  15. Shui, J.W. et al. (2012) Nature 488:222.
  16. Montgomery, R.I. et al. (1996) Cell 87:427.
Long Name
Herpesvirus Entry Mediator
Entrez Gene IDs
8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names
ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2

Citation for Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors
    Authors: H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka
    PLoS Pathog, 2017-01-03;13(1):e1006120.
    Applications: Bead-based Bioassay

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