Home / ICAM-5 / Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

Catalog #: 1173-M5
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1173-M5-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (5)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ICAM-5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant mouse ICAM-5 Fc Chimera coated plates (25 µg/mL with 100 µL/well), >25% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse ICAM-5 protein
Mouse ICAM-5
Leu31-Arg828 (Pro47Arg)
Accession # Q60625.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q60625.1
N-terminal Sequence
Analysis
Leu31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
112 kDa (monomer)
SDS-PAGE
166 kDa, reducing conditions

Product Datasheets

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1173-M5

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1173-M5

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM-5 is a member of the immunoglobulin superfamily and shares 38 - 55% amino acid homology with other ICAMs. Structurally, ICAM-5 contains nine Ig domains that included 15 N‑glycosylation sites, a single transmembrane region, and C-terminal cytoplasmic tail (1).

As with other members of the ICAM family, ICAM-5 has been shown to be involved in cellular adhesion. ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of ICAM-5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4 - 5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

References
  1. Tian, L. et al. (2000) Eur. J. Immunol. 30:810.
  2. Mizuno, T. and K. Mori (1997) J. Biol. Chem. 272:1156.
  3. Tian, L. et al. (2000) J. Cell Biol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Entrez Gene IDs
7087 (Human); 15898 (Mouse)
Alternate Names
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin

Citations for Recombinant Mouse ICAM-5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia
    Authors: S Paetau, T Rolova, L Ning, CG Gahmberg
    Front Mol Neurosci, 2017-12-22;10(0):431.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Vitronectin induces phosphorylation of ezrin/radixin/moesin actin-binding proteins through binding to its novel neuronal receptor telencephalin.
    Authors: Furutani Y, Kawasaki M, Matsuno H, Mitsui S, Mori K, Yoshihara Y
    J Biol Chem, 2012-09-27;287(46):39041-9.
    Applications: Surface Plasmon Resonance
  3. MMPs and soluble ICAM-5 increase neuronal excitability within in vitro networks of hippocampal neurons.
    Authors: Niedringhaus M, Chen X, Dzakpasu R, Conant K
    PLoS ONE, 2012-08-13;7(8):e42631.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
    Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
    J. Neurochem., 2011-01-19;118(4):521-32.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Matrix metalloproteinase-dependent shedding of intercellular adhesion molecule-5 occurs with long-term potentiation.
    Authors: Conant K, Wang Y, Szklarczyk A, Dudak A, Mattson MP, Lim ST
    Neuroscience, 2010-01-04;166(2):508-21.
    Applications: Enzyme Assay Substrate

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