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Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF

Catalog #: 1026-GR
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1026-GR-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IFN-gamma -mediated anti-viral activity using L‑929 mouse fibroblast cells. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 6-36 ng/mL in the presence of 1 ng/mL of recombinant mouse IFN-gamma.
Source
Mouse myeloma cell line, NS0-derived mouse IFN-gamma R1/CD119 protein
Mouse IFN-gamma R1
(Ala26-Asp253)
Accession # Q91Y85		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q91Y85
N-terminal Sequence
Analysis
Ala26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52.4 kDa (monomer)
SDS-PAGE
70-90 kDa, reducing conditions

Product Datasheets

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1026-GR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1026-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma  R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

References
  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.
Long Name
Interferon gamma Receptor 1
Entrez Gene IDs
3459 (Human); 15979 (Mouse)
Alternate Names
AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

Citations for Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A RIPK2 inhibitor delays NOD signalling events yet prevents inflammatory cytokine production.
    Authors: Nachbur U, Stafford C, Bankovacki A, Zhan Y, Lindqvist L, Fiil B, Khakham Y, Ko H, Sandow J, Falk H, Holien J, Chau D, Hildebrand J, Vince J, Sharp P, Webb A, Jackman K, Muhlen S, Kennedy C, Lowes K, Murphy J, Gyrd-Hansen M, Parker M, Hartland E, Lew A, Huang D, Lessene G, Silke J
    Nat Commun, 2015-03-17;6(0):6442.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Importance of neonatal FcR in regulating the serum half-life of therapeutic proteins containing the Fc domain of human IgG1: a comparative study of the affinity of monoclonal antibodies and Fc-fusion proteins to human neonatal FcR.
    Authors: Suzuki T, Ishii-Watabe A, Tada M, Kobayashi T, Kanayasu-Toyoda T, Kawanishi T, Yamaguchi T
    J. Immunol., 2010-01-18;184(4):1968-76.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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