Recombinant Mouse IL-10 (HEK293-Expressed) Protein, CF

Catalog #: 11404-ML Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11404-ML-020
11404-ML-050
11404-ML-100
11404-ML-250

Recombinant Mouse IL‑10 (HEK293-Expressed) Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-10 (HEK293-Expressed) Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED₅₀ for this effect is 0.0650-0.780 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse IL-10 protein
Gln22-Ser178

Accession #

P18893.2

N-terminal Sequence
Analysis

Gln22 inferred from enzymatic pyroglutamate treatment revealing Tyr23.

Predicted Molecular Mass

19 kDa

SDS-PAGE

16-21 kDa, under reducing conditions.

Product Datasheets

11404-ML

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11404-ML

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IL‑10 (HEK293-Expressed) (Catalog # 11404-ML) stimulates cell proliferation of the MC/9‑2 mouse mast cell line. The ED₅₀ for this effect is 0.0650‑0.780 ng/mL.

Reconstitution Calculator

Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References

Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
Hsu, D.-H. et al. (1990) Science 250:830.
Moore, K. et al. (1990) Science 248:1230.
Windsor, W.T. et al. (1993) Biochemistry 32:8807.
Syto, R. et al. (1998) Biochemistry 37:16943.
Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF