Recombinant Mouse IL-11 Protein, CF Summary
Product Specifications
Pro22-Leu199
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11585-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose. |
| Reconstitution | Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
Recombinant Mouse IL-11 Protein (Catalog # 11585-IL) stimulates T11 mouse plasmacytoma cell proliferation. The ED50 for this effect is less than 0.750 ng/mL.
View Larger
2 μg/lane of Recombinant Mouse IL‑11 Protein (Catalog # 11585-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 20 kDa under reducing conditions.
Reconstitution Calculator
Background: IL-11
IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-4). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The mouse IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature mouse IL-11 shares 88%, 97%, and 89% aa sequence identity with human, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (5). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (6-8).
- Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
- Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
- Kawashima, I. et al. (1991) FEBS Lett. 283:199.
- Morris, J.C. et al. (1996) Exp. Hematol. 24:1369.
- Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
- Curtis, D.J. et al. (1997) Blood 90:4403.
- Baumann, H. et al. (1996) J. Immunol. 157:284.
- Karow, J. et al. (1996) Biochem. J. 318:489.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse IL-11 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse IL-11 Protein, CF and earn rewards!
Have you used Recombinant Mouse IL-11 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
