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Recombinant Mouse IL-17A (CHO-expressed) Protein

Catalog #: 7956-ML
5 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
7956-ML-025/CF
7956-ML-010/CF
7956-ML-100/CF

With Carrier

Catalog # Availability Size / Price Qty
7956-ML-025
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Product Details
Citations (5)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-17A (CHO-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1.25 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse IL-17/IL-17A protein
Ala26-Ala158
Accession #
Q62386
N-terminal Sequence
Analysis
Ala26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15 kDa (monomer)
SDS-PAGE
15 -22 kDa, reducing conditions

Product Datasheets

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7956-ML (with carrier)

Product Datasheet
COA
SDS

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7956-ML/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7956-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7956-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL‑17A shares 61% and 89% amino acid sequence identity with human and rat IL‑17A, respectively (3, 4). IL‑17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD (7, 8). Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (17, 18).

References
  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Yao, Z. et al. (1996) Gene 168:223.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.
  5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  8. Rong, Z. et al. (2009) Cell Res. 19:208.
  9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
  10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
  11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
  13. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  14. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
  15. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
  16. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
  17. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
  18. Kryczek, I. et al. (2009) Blood 114:357.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Citations for Recombinant Mouse IL-17A (CHO-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Glia-derived adenosine in the ventral hippocampus drives pain-related anxiodepression in a mouse model resembling trigeminal neuralgia
    Authors: Lv, XJ;Lv, SS;Wang, GH;Chang, Y;Cai, YQ;Liu, HZ;Xu, GZ;Xu, WD;Zhang, YQ;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay
  2. Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis
    Authors: Wayne, CR;Bremner, L;Faust, TE;Dur�n-Laforet, V;Ampatey, N;Ho, SJ;Feinberg, PA;Arvanitis, P;Ciric, B;Ruan, C;Elyaman, W;Delaney, SL;Vargas, WS;Swedo, S;Menon, V;Schafer, DP;Cutforth, T;Agalliu, D;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
    Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
    Nature Immunology, 2022-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway
    Authors: EM Byrne, M Llorián-Sa, M Tang, A Margariti, M Chen, H Xu
    Biomedicines, 2021-07-16;9(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy
    Authors: Y Wang, S Gao, S Gao, N Li, B Xie, X Shen
    Cell & bioscience, 2021-05-01;11(1):82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse IL-17A (CHO-expressed) Protein
By Anonymous on 06/16/2020
Application: In vivo study