Recombinant Mouse IL-22BP His-tag Protein, CF Summary
Product Specifications
Thr19-Pro230, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9597-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Mouse IL-22BP (Catalog # 9597-BP) inhibits IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is 2-12 ng/mL.
Reconstitution Calculator
Background: IL-22BP
Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 Rb. IL-10 Rb is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1, 2). IL-22BP blocks the interaction of IL-22 with IL-22 R, preventing IL-22 induced production of reactive oxygen species, IL-6, IL-10, and TNF-a (3-8). In vivo, it regulates the proinflammatory effects of IL-22 (e.g. neutrophil infiltration) but not of IL-10 (7). Mouse IL-22BP can neutralize the bioactivity of both mouse and human IL-22 (6). IL-22BP is produced by dendritic cells (DE), epithelial cells, activated B cells, and activated monocytes (3, 6, 9, 10). It is constitutively expressed by DC but is down-regulated during local inflammation and in response to tissue damage (11-13). IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). Mature mouse IL-22BP consists of two tandem Fibronectin type III domains and shares 85% and 68% amino acid sequence identity with rat IL-22BP and the short isoform of human IL-22BP, respectively (6, 14).
- Lim, C. and R. Savan (2014) Cytokine Growth Factor Rev. 25:257.
- Mizoguchi, A. (2012) Inflamm. Bowel Dis. 18:1777.
- Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
- Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
- Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
- Wei, C.-C. et al. (2003) Genes Immun. 4:204.
- Weber, G.F. et al. (2007) Infect. Immun. 75:1690.
- Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
- Lecart, S. et al. (2002) Int. Immunol. 14:1351.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
- Wolk, K. et al. (2007) J. Immunol. 178:5973.
- Huber, S. et al. (2012) Nature 491:259.
- Martin, J.C.J. et al. (2014) Mucosal Immunol. 7:101.
- Weiss, B. et al. (2004) Genes Immun. 5:330.
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