Home / IL-36 alpha/IL-1F6 / Recombinant Mouse IL-36 alpha/IL-1F6 Protein

Recombinant Mouse IL-36 alpha/IL-1F6 Protein

Catalog #: 2297-ML
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
2297-ML-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2297-ML-025
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Recombinant Mouse IL-36 alpha/IL-1F6 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse IL-36 alpha at 1 µg/mL (100 µL/well) can bind recombinant mouse IL-1 Rrp2 (Catalog # 2354-RP) with a linear range of 0.15 ‑ 5 µg/mL.
Source
E. coli-derived mouse IL-36 alpha/IL-1F6 protein
Met1-His160
Accession #
NP_062323
N-terminal Sequence
Analysis
Met1
Predicted Molecular Mass
21 kDa

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2297-ML (with carrier)

Product Datasheet
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SDS

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2297-ML/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2297-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2297-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-36 alpha/IL-1F6

Mouse  interleukin‑36 alpha [IL‑36 alpha ; previously IL‑1F6 and also named FIL‑1 epsilon (epsilon)] is a member of the IL‑1 family of proteins (1 ‑ 3, 7). IL‑1 family members include IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36Ra/IL‑1F5, IL‑37/IL‑1F7, IL‑36 beta /IL‑1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10 (4, 7). All family members show a 12 beta ‑strand, beta ‑trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL‑36 alpha /IL‑1F6 is synthesized as a 160 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation site(s) (2, 5). It appears to be actively secreted (1). When found in cell lysates, it presents as an 18 kDa monomer (2). Mouse to human, full length IL‑36 alpha /IL‑1F6 has 54% aa identity. Within the family, IL‑36 alpha /IL‑1F6 is 29% aa identical to IL‑1ra, and 32%, 32%, 34%, 41%, and 28% aa identical to IL‑1 beta, IL‑36Ra/IL‑1F5, IL‑36 beta /IL‑1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL‑36 alpha /IL‑1F6 include monocytes, B cells and T cells (1, 4). Notably, IL‑36 alpha /IL‑1F6 is the only novel IL‑1 family member found to be expressed on T cells. The receptor for IL‑36 alpha /IL‑1F6 is reported to be a combination of IL‑1 Rrp2 and IL‑1 RAcP (6). Recombinant IL‑36 alpha /IL‑1F6, along with IL‑36 beta /IL‑1F8 and IL‑36 gamma /IL‑1F9, has been shown to act as an agonist by activating the pathway involving NF‑ kappa B and MAPK in an IL‑1 Rrp2 dependent manner. This suggests that IL‑36 alpha /IL‑1F6 may signal in similar fashion to IL‑1 and IL‑18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Debets, R. et al. (2001) J. Immunol. 167:1440.
  6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  7. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Entrez Gene IDs
27179 (Human); 54448 (Mouse)
Alternate Names
FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha

Citation for Recombinant Mouse IL-36 alpha/IL-1F6 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Externalization of the leaderless cytokine IL-1F6 occurs in response to lipopolysaccharide/ATP activation of transduced bone marrow macrophages.
    Authors: Martin U, Scholler J, Gurgel J, Renshaw B, Sims JE, Gabel CA
    J. Immunol., 2009-08-28;183(6):4021-30.
    Applications: Western Blot

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