Recombinant Mouse IL-9 (HEK293 expressed) Protein, CF

Catalog #: 11391-ML Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11391-ML-010
11391-ML-050
11391-ML-250

Recombinant Mouse IL‑9 (HEK293 expressed) Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-9 (HEK293 expressed) Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED₅₀ for this effect is 1.50-15.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse IL-9 protein
Gln19-Pro144

Accession #

P15247.1

N-terminal Sequence
Analysis

Gln19 (Blocked), reveal Ala20 after deblocking

Predicted Molecular Mass

14 kDa

SDS-PAGE

35-39 kDa, under reducing conditions.

Product Datasheets

11391-ML

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11391-ML

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IL‑9 (HEK293 expressed) Protein (Catalog # 11391-ML) induces MO7e human megakaryocytic leukemic cell proliferation. The ED₅₀ for this effect is 1.50‑15.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IL‑9 (HEK293 expressed) Protein (Catalog # 11391-ML) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-39 kDa, under reducing conditions.

Reconstitution Calculator

Background: IL-9

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4⁺ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively (14).

References

Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
Townsend, M.J. et al. (2000) Immunity 13:573.
Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
Tan, C. et al. (2010) J. Immunol. 185:6795.
Jager, A. et al. (2009) J. Immunol. 183:7169.
Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
Lu, L.-F. et al. (2006) Nature 442:997.
Stassen, M. et al. (2000) J. Immunol. 164:5549.
Van Snick, J. et al. (1989) J. Exp. Med. 169:363.

Long Name

Interleukin 9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Alternate Names

Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40