Recombinant Mouse IL-Y Protein, CF

Catalog #: 9989-IL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9989-IL-050

Recombinant Mouse IL-Y Protein Bioactivity

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All IL-Y (IL-12p40/IL-27p28) Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-Y Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-12 induced IFN-gamma secretion by mouse splenocytes. The ED₅₀ for this effect is 10-60 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse IL-Y (IL-12p40/IL-27p28) protein

Mouse IL-12p40 (Met23-Ser335) Accession # P43432	GGGSGGGSGGGS	Mouse IL-27p28 (Phe29-Ser234) Accession # Q8K3I6
N-terminus		C-terminus

Accession #

P43432, Q8K3I6

N-terminal Sequence
Analysis

Met23 (IL-12p40)

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

60 kDa

SDS-PAGE

74-84 kDa, reducing conditions

Product Datasheets

9989-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9989-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IL-Y (Catalog # 9989-IL) inhibits IL‑12 induced IFN-gamma secretion in mouse splenocytes. The ED₅₀ for this effect is 10‑60 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IL-Y was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-84 kDa.

Reconstitution Calculator

Background: IL-Y (IL-12p40/IL-27p28)

IL-Y is a new member of the IL-12 cytokine family consists of IL-12p40 and IL-27p28 subunits (1, 2, 3). The mature mouse IL-12p40 subunit contains 312 amino acids and shares 66% and 93%amino acid (aa) identity with human and rat IL-12p40, respectively. The mature mouse IL-27p28 subunit contains 205 amino acids and shares 70% and 89% aa identity with human and rat IL-27p28, respectively. IL-Y inhibits the differentiation and inflammatory responses of Th1 and Th17 cells while promoting expansion of IL-10 and Foxp3 expressing regulatory T cells (3). Treatment of prediabetic non-obese diabetic mice using adenovirus vector expressing IL-Y prevents the onset of hyperglycemia with reduced expression of inflammatory mediators such as IFN-gamma. IL-Y significantly stimulates a unique cytokine and chemokine expression profile as well as to activate STAT3, in part, through a pathway involving WSX-1 (2).

References