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Recombinant Mouse IL1RAPL2 Fc Chimera Protein, CF

Catalog #: 11222-MR Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11222-MR-050
Recombinant Mouse IL1RAPL2 Fc Chimera Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL1RAPL2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL1RAPL2 Fc Chimera (Catalog # 11222-MR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) binds with an ED50 of 20.0-240 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL1RAPL2 protein
Mouse IL1RAPL2
(Thr17-Lys354)
Accession # Q9ERS6.1		IEGRMDPMouse IgG2A
(Glu98-Lys330)
N-terminusC-terminus
Accession #
Q9ERS6.1
N-terminal Sequence
Analysis
Thr17
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66 kDa
SDS-PAGE
75-88 kDa, under reducing conditions.

Product Datasheets

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11222-MR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11222-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Mouse IL1RAPL2 Fc Chimera Protein (Catalog # 11222-MR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera Protein (9995-PR) binds with an ED50 of 20.0-240 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse IL1RAPL2 Fc Chimera Protein (Catalog # 11222-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-88 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL1RAPL2

The Interleukin 1 receptor family (IL-1R) comprises at least eleven members including IL-1 RI (IL-1R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL‑1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 R beta (AcPL/IL-1 R7), IL1RAPL1 (TIGIRR-2/IL-1 R8), and IL1RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL1RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Human IL1RAPL2 is a type I transmembrane glycoprotein with a 16 aa signal peptide, a 338 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 311 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL1RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1RAPL1 and SIGIRR but not other family members (3). Human and mouse IL1RAPL2 share approximately 95% aa sequence identity. Human IL1RAPL2 is most homologous (63%) to IL-1RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL1RAPL2 has not been identified (1).

References
  1. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  2. Andre, R. et al. (2005) J. Neurochem. 95:324.
  3. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
  4. Jin, H. et al. (2000) Eur. J. Hum. Genet. 8:87.
  5. Carrie, A. et al. (1999) Nat. Genet. 23:25.
Long Name
Interleukin 1 Receptor Accessory Protein-like 2
Entrez Gene IDs
26280 (Human); 60367 (Mouse)
Alternate Names
IL-1R10; IL-1R-9; IL1R9IL-1 receptor accessory protein-like 2; IL-1R9IL-1RAPL-2; IL1RAPL2; IL-1RAPL2; IL-1-RAPL-2; IL1RAPL-2IL1RAPL-2-related protein; interleukin 1 receptor 9; interleukin 1 receptor accessory protein-like 2; Three immunoglobulin domain-containing IL-1 receptor-related 1; TIGIRR-1; TIGIRR-1Interleukin-1 receptor 9; X-linked interleukin-1 receptor accessory protein-like 2

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