Recombinant Mouse JAM-B/VE-JAM Fc Chimera Protein, CF

Catalog #: 988-VJ
7 Citations Datasheet / COA / SDS

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988-VJ-050

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Citations (7)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse JAM-B/VE-JAM Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes. Liang, T.W. et al. (2002) J. Immunol. 168:1618.

When Recombinant Mouse JAM-B Fc Chimera is immobilized on goat anti-human IgG Fc antibody coated wells, J45.01 cells (100 µL/well at 10⁶ cells/mL) adhesion is induced in a dose dependent manner after a 2 hour incubation at 37 °C. The ED₅₀ for this effect is 10-60 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse JAM-B/VE-JAM protein

Mouse JAM-B (Phe29 - Asn236) Accession #Q9JI59	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

Q9JI59

N-terminal Sequence
Analysis

Phe29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

64-70 kDa, reducing conditions

Product Datasheets

988-VJ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

988-VJ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: JAM-B/VE-JAM

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (2, 3, 5). Mouse JAM-B/VE-JAM cDNA predicts a 298 amino acid (aa) residue precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (2, 3). Mouse JAM-B shares approximately 79% aa sequence homology with its human homologue. It also shares approximately 35% aa sequence homology with mouse JAM-A or JAM-C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM-C, but not JAM-A (4, 5, 7). It is also a ligand for the integrin alpha4beta1. However, the JAM‑B/alpha4beta1 interaction is facilitated only after prior adhesion of JAM-B to JAM-C (6). Through its heterotypic interactions with JAM-C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).

References

Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
Aurand-Lions, M. et al. (2001) Blood 98:3699.
Palmeri, A. et al. (2000) J. Biol. Chem. 275:19139.
Cunnigham, S. et al. (2000) J. Biol. Chem. 275:34750.
Liang, T. et al. (2002) J. Immunol. 168:1618.
Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
Arrate, M. et al. (2001) J. Biol. Chem. 276:45826.

Long Name

Junctional Adhesion Molecule B

Entrez Gene IDs

58494 (Human); 67374 (Mouse)

Alternate Names

C21orf43; CD322 antigen; CD322; JAM2; JAMB; JAM-B; JAM-BJAM-IT/VE-JAM; junctional adhesion molecule 2JAM-2; junctional adhesion molecule B; PRO245; Vascular endothelial junction-associated molecule; VE-JAM; VE-JAMVEJAMCD322

Related Research Areas

Citations for Recombinant Mouse JAM-B/VE-JAM Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Transcriptional and Post-Translational Regulation of Junctional Adhesion Molecule-B (JAM-B) in Leukocytes under Inflammatory Stimuli
Authors: PE Day-Walsh, B Keeble, G Pirabagar, SJ Fountain, PA Kroon
International Journal of Molecular Sciences, 2022-08-03;23(15):.
Species: Mouse
Sample Types: Control
Applications: Western Blot
Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis
Authors: V Radulovic, M van der Ga, S Koide, V Sigurdsson, S Lang, S Kaneko, K Miharada
Cell Rep, 2019-06-04;27(10):2826-2836.e5.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis
Authors: E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen
Cell Adh Migr, 2016-04-25;10(4):419-33.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Function of Jam-B/Jam-C interaction in homing and mobilization of human and mouse hematopoietic stem and progenitor cells.
Authors: Arcangeli M, Bardin F, Frontera V, Bidaut G, Obrados E, Adams R, Chabannon C, Aurrand-Lions M
Stem Cells, 2014-04-01;32(4):1043-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF.
Authors: Su RW, Jia B, Ni H
PLoS ONE, 2012-04-12;7(4):e34325.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
Blood, 2011-08-25;118(17):4609-19.
Species: Mouse
Sample Types: Whole Cells
Applications: Adhesion Assay, Flow Cytometry
Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
Immunology, 2009-10-01;128(2):196-205.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay