Recombinant Mouse Kirrel1/NEPH1 Protein, CF Summary
Product Specifications
Leu48-Leu525, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5030-K1
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Kirrel1/NEPH1
Kirrel1, also called neph1, is a 90 - 110 kDa type I transmembrane glycoprotein that belongs to the NEPH family of the immunoglobulin superfamily (1 - 4). The 789 amino acid (aa) mouse Kirrel1 contains a 47 aa signal sequence, a 484 aa extracellular domain (ECD) with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 237 aa cytoplasmic domain (5). An isoform diverges in the cytoplasmic domain and ends at aa 634, prior to four tyrosine phosphorylation sites, a podocin interaction motif, and a C-terminal PDZ motif that are all important for the signaling functions of Kirrel1 (1, 6). If expressed, this isoform would be expected to block homo- and hetero-multimer formation, thus acting as an inhibitor. The ECD also contains a site for FGF/FGF R interaction, and an RGD site that may allow integrin-mediated cell attachment (5). Mouse Kirrel1 shares 99% and 97% aa identity with rat and human Kirrel1, respectively, within the ECD. Kirrel1 expression has been mainly studied in the kidney glomerular slit diaphragm, but its expression with nephrin or other family members has also been reported in central nervous system neurons, pancreas and placenta (3, 4, 7 - 9). Kirrel1 forms cis hetero-oligomers with nephrin, which brings together signaling molecules that direct actin polymerization (3, 4, 10). This interaction is essential for barrier function in the slit diaphragm, and mice deleted for Kirrel1 die perinatally due to proteinuria and failure to thrive (2, 3).
- Sellin, L. et al. (2003) FASEB J.17:115.
- Donoviel, D.B. et al. (2001) Mol. Cell. Biol. 21:4829.
- Liu, G. et al. (2003) J. Clin. Invest. 112:209.
- Barletta, G-M. et al. (2003) J. Biol. Chem. 278:19266.
- Swissprot Accession # Q96J84.
- Huber T.B. et al. (2003) J. Biol. Chem. 278:13417.
- Gerke, P. et al. (2006) J. Comp. Neurol. 498:466.
- Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
- Beall, M.H. et al. (2005) J. Soc. Gynecol. Investig. 12:298.
- Garg, P. et al. (2007) Mol. Cell. Biol. 27:8698.
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