Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF Summary
Product Specifications
| Mouse LRP-6 (Ala20-Ile628) Accession # NP_032540 | IEGRMDP | Mouse IgG2a (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9950-LR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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Scientific Data
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2 μg/lane of Recombinant Mouse LRP‑6 N-Terminal Fragment Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 100-120 kDa and 200-220 kDa, respectively.
Reconstitution Calculator
Background: LRP-6
The low-density lipoprotein (LDL) receptor-related protein 6 (LRP-6), along with LRP-5, constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins and mediate the down-regulation of GSK-3 activity and the initiation of the canonical Wnt/beta-catenin signaling cascade (1, 2). Mouse LRP-6 consists of a 19 amino acid (aa) signal sequence, a 1351 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 20 aa cytoplasmic domain (3). The LRP-6 ECD is composed of four distinct YWTD beta -propeller (BP) motifs followed by three LDLR type A repeats (4). The first two LRP-6 BP motifs bind Wnt-1, -2, -2b, -6, -8a, -9a, -9b, and -10b, while the third and fourth BP motifs bind Wnt-3a (5-10). This product includes the first and second BP motifs of mouse LRP-6 (aa 20-628) that share 98% aa sequence identity with the first two BP motifs of rat and human LRP-6. Dkk-1, a known antagonist of Wnt signaling, interacts with LRP-6 as a bipartite inhibitor, binding the first and third BP motifs of LRP-6 simultaneously (8, 11). Mice lacking LRP-6 die at birth with a truncation of the axial skeleton, limb defects, microphthalmia, and malformation of the urogenital system (2). Additionally, LRP-6 may be essential for osteoblast differentiation during bone remodeling and, along with LRP-5, is required for postnatal bone development (12, 13).
- Joiner, D.M. et al. (2013) Trends Endocrinol. Metab. 24:31.
- Pinson, K.I. et al. (2000) Nature 407:535.
- Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
- He, X. et al. (2004) Development 131:1663.
- Ai, M. et al. (2005) Mol. Cell. Biol. 25:4946.
- Gong, Y. et al. (2010) PLoS One 5:e12682.
- Itasaki, N. et al. (2003) Development 130:4295.
- Mao, B. et al. (2001) Nature 411:321.
- Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
- Bourhis, E. et al. (2010) J. Biol. Chem. 285:9172.
- Ahn, V.E. et al. (2011) Dev. Cell 21:862.
- Li, C. et al. (2013) J. Bone Miner. Res. 28:2094.
- Riddle, R.C. et al. (2013) PLoS One 8:e63323.
Citation for Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Human cell surface-AAV interactomes identify LRP6 as blood-brain barrier transcytosis receptor and immune cytokine IL3 as AAV9 binder
Authors: Shay, TF;Jang, S;Brittain, TJ;Chen, X;Walker, B;Tebbutt, C;Fan, Y;Wolfe, DA;Arokiaraj, CM;Sullivan, EE;Ding, X;Wang, TY;Lei, Y;Chuapoco, MR;Chou, TF;Gradinaru, V;
Nature communications
Species: N/A
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR)
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