Recombinant Mouse MAGP-2/MFAP5 Protein, CF Summary
Product Specifications
When 2 x 104 cells/well are added to Recombinant Mouse MAGP-2/MFAP5 coated plates (10 μg/mL with 100 μL/well), approximately 45-70% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determinded by each laboratory for each application.
Gln29-Leu164 with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7345-MG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: MAGP-2/MFAP5
MAGP-2 (Microfibril-associated glycoprotein 2), also called MFAP5 (microfibrillar associated protein 5) is a secreted, cell-associated 25 kDa mammalian member of the MFAP family of proteins (1). Mouse MAGP-2 cDNA encodes 164 amino acids (aa) including a 28 aa signal sequence, an RDG motif (aa 30 ‑ 32; absent in MAGP-1) and a central cysteine‑rich binding domain (aa 76 ‑ 133) (1 ‑ 3). Mature mouse MAGP-2 shares 86%, 96%, 84%, 85% and 85% aa identity with human, rat, bovine, porcine and canine MAGP-2, respectively. Both MAGP-1 and MAGP-2 bind and covalently crosslink Fibrillin-1 and -2 through the Cys-rich binding domain. MAGP-2, however, has a more limited distribution and binds a more limited set of extracellular matrix proteins (1 ‑ 3). This may suggest a specialized role in microfibril biology. Functionally, the MAGP-2 RGD motif binds to the integrin alpha v beta 3, allowing cell attachment to microfibrils (4). MAGP‑2 is thought to facilitate microfibril assembly and induce elastin fiber formation (1 ‑ 3, 5). Through its binding to EGF repeats, MAGP‑2 mediates the metalloproteinase-dependent release of Jagged1, and the metalloproteinase-independent release of Notch extracellular domains (6, 7). In endothelial cells, MAGP-2 promotes angiogenic sprouting by inhibiting Notch signaling, by binding to integrin alpha v beta 3 (which then enhances VEGF signaling), and/or enhancing the endothelial cell response to FGF basic and EGF (8, 9). In ovarian cancer, MAGP-2 expression is associated with cancer cell survival, increased microvessel density, and poor prognosis (10). Skin MAGP-2 expression is increased in human scleroderma and the tight skin (TSK) mouse and may contribute to abnormal collagen accumulation in these conditions by increasing the half-life of type I collagen (11, 12). Mutations within the MAGP-2-binding region of human Fibrillin-1 can be found in Marfan syndrome (5).
- Gibson, M.A. et al. (1996) J. Biol. Chem. 271:1096.
- Hanssen, E. et al. (2004) J. Biol. Chem. 279:29185.
- Segade, F. et al. (2002) J. Biol. Chem. 277:11050.
- Gibson, M.A. et al. (1999) J. Biol. Chem. 274:13060.
- Lemaire, R. et al. (2007) J. Biol. Chem. 282:800.
- Nehring, L.C. et al. (2005) J. Biol. Chem. 280:20349.
- Miyamoto, A. et al. (2006) J. Biol. Chem. 281:10089.
- Albig, A.R. et al. (2008) Microvasc. Res. 76:7.
- Albig, A.R. et al. (2007) Angiogenesis 10:197.
- Mok, S.C. et al. (2009) Cancer Cell 16:521.
- Lemaire, R. et al. (2005) Arthritis Rheum. 52:1812.
- Ito, S. et al. (2006) Am.J. Respir. Cell Mol. Biol. 34:688.
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