Recombinant Mouse Mill2 Fc Chimera Protein, CF

Catalog #: 7657-ML Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7657-ML-050

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Recombinant Mouse Mill2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of EL‑4 mouse lymphoblast cells. The ED₅₀ for this effect is 1-5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Mill2 protein

Mouse Mill2 (Ser30-His298) Accession # NP_715641	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_715641

N-terminal Sequence
Analysis

Ser30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

57.6 kDa (monomer)

SDS-PAGE

60-66 kDa, reducing conditions

Product Datasheets

7657-ML

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7657-ML

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Mill2

Mill2 (MHC class I‑like located near the leukocyte receptor complex‑2) is an ~41 kDa glycosyl‑phosphatidylinositol (GPI)‑linked glycoprotein that is a member of the non‑classical MHC class I‑like family (1‑3). Mouse Mill2 cDNA encodes 340 amino acids (aa) that include a signal sequence, an MHC I domain containing alpha 1, alpha 2 and alpha 3 regions, and a GPI anchor sequence (1). A long form contains 15 aa inserted near the N‑terminus. Mature mouse Mill2 (short form) shares 74% aa sequence identity with rat Mill2 and approximately 61% aa identity with mouse Mill1. Human orthologs of Mill proteins have not been identified, although similarities in structure and sequence with human HFE and MICA/B have been noted (1, 4, 5). Mill2 is expressed on proliferating thymocytes, smooth muscle cells and fibroblasts in skin, bladder, uterus and colonic muscularis (5). Low expression is detected in many tumor cell lines (5). Mill proteins bind beta 2‑microglobulin (2). While Mill1 requires beta 2‑microglobulin for surface expression, the short form of Mill2 does not, and surface expression of the long form is higher in its presence (5). Mill proteins are unlikely to present peptides and do not bind to NKG2D (2, 5). Recombinant Mill2 has, however, shown pH‑dependent binding to TfR/CD71‑expressing immature cells in the B lymphocyte and erythrocyte lineages, as well as activated, proliferating splenic T and B cells and some cell lines (5).

References