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Recombinant Mouse MMR/CD206 Protein

Catalog #: 2535-MM
6 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
2535-MM-050/CF

With Carrier

Catalog # Availability Size / Price Qty
2535-MM-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse MMR/CD206 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Mannan is coated at 0.2 μg/mL (100 μL/well), the concentration of Recombinant Mouse MMR/CD206 that produces 50% of the optimal binding response is approximately 0.1-0.6 μg/mL..
Source
Mouse myeloma cell line, NS0-derived mouse MMR/CD206 protein
Leu19-Ala1388, with a C-terminal 6-His tag
Accession #
Q2HZ94
N-terminal Sequence
Analysis
Leu19
Predicted Molecular Mass
156 kDa (monomer)
SDS-PAGE
180 kDa, reducing conditions

Product Datasheets

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2535-MM (with carrier)

Product Datasheet
COA
SDS

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2535-MM/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2535-MM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2535-MM/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) mouse CD206 precursor contains a signal sequence (19 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The mouse CD206 ECD shares 96% aa sequence identity with rat MR, and 83‑84% with human, equine, porcine and canine CD206. The cysteine-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewisa and ‑Lewisx (1, 7, 9). The FNII domain mediates Ca2+‑independent binding of collagens (2, 10). The CTLDs participate in Ca2+‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

References
  1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
  2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
  3. Harris, N. et al. (1992) Blood 80:2363.
  4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
  5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.
  6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
  8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
  9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
  11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
  12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.
Long Name
Macrophage Mannose Receptor
Entrez Gene IDs
4360 (Human); 17533 (Mouse); 291327 (Rat)
Alternate Names
CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1

Citations for Recombinant Mouse MMR/CD206 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Sensitivity of Kupffer cells and liver sinusoidal endothelial cells to ricin toxin and ricin toxin-Ab complexes
    Authors: B Mooney, FJ Torres-Vel, J Doering, DJ Ehrbar, NJ Mantis
    J. Leukoc. Biol., 2019-07-16;0(0):.
    Species: Plant - Ricinus communis
    Sample Types: Protein
    Applications: Bioassay
  2. Mannosylated Polyrotaxanes for Increasing Cellular Uptake Efficiency in Macrophages through Receptor-Mediated Endocytosis
    Authors: K Shibaguchi, A Tamura, M Terauchi, M Matsumura, H Miura, N Yui
    Molecules, 2019-01-26;24(3):.
    Species: N/A
    Sample Types: Polymer
    Applications: Surface Plasmon Resonance, Surface Plasmon Resonance (SPR
  3. Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival
    Authors: K Subramania, DR Neill, HA Malak, L Spelmink, S Khandaker, G Dalla Libe, E Dearing, A Kirby, M Yang, A Achour, J Nilvebrant, PÅ Nygren, L Plant, A Kadioglu, B Henriques-
    Nat Microbiol, 2018-11-12;0(0):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Precision Targeting of Tumor Macrophages with a CD206 Binding Peptide
    Authors: P Scodeller, L Simón-Grac, S Kopanchuk, A Tobi, K Kilk, P Säälik, K Kurm, ML Squadrito, VR Kotamraju, A Rinken, M De Palma, E Ruoslahti, T Teesalu
    Sci Rep, 2017-11-07;7(1):14655.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
    PLoS ONE, 2015-03-18;10(3):e0119244.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis.
    Authors: Green RS, Stone EL, Tenno M, Lehtonen E, Farquhar MG, Marth JD
    Immunity, 2007-08-02;27(2):308-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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