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Recombinant Mouse MuSK His-tag Protein, CF

Catalog #: 11519-MK Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11519-MK-050
Recombinant Mouse MuSK His-tag Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse MuSK His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Mouse MuSK His-tag binds to Recombinant Mouse LRP-4 His-tag (Catalog # 10229-LR) and Agrin His-tag with a ED50 of 0.0800‑0.800 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse MuSK protein
Glu22-Thr494, with a C-terminal 6-His tag
Accession #
Q61006.1
N-terminal Sequence
Analysis
Glu22
Predicted Molecular Mass
53 kDa
SDS-PAGE
63-69 kDa, under reducing conditions.

Product Datasheets

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11519-MK

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11519-MK

Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity View Larger

Recombinant Mouse MuSK His-tag Protein (Catalog # 11519-MK) binds to Recombinant Mouse LRP-4 His-tag Protein (10229-LR) and Agrin His-tag Protein with an ED50 of 0.0800-0.800 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse MuSK His-tag Protein (Catalog # 11519-MK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63 - 69 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MuSK

MuSK (muscle-specific kinase) is a 100-kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). MuSK contains a 473 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain. Human MuSK has multiple isoforms. One contains deletions at residues 307-394 and 454-461, while a second is a short soluble form that contains residues 120­-209 plus a unique 24 aa C­terminal tail (3). Within the ECD, mouse MuSK shares 91% and 96% aa sequence identity with human and rat MuSK, respectively. MuSK is a crucial signaling molecule in the formation of the neuromuscular junction(NMJ). Defects in MuSK causes muscle weakness in congenital myathenic syndromes (4). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (5), Wnt ligands (6), Biglycan (7), ColQ (8) and BMPs(10). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (8, 9). When agrin binds to the N-terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (11, 12).

References
  1. Valenzuela, D.M. et al. (1995) Neuron 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. DeChiara, T. et al. (1996) Cell 85:501.
  5. Kim, N. et al. (2008) Cell 135:334.
  6. Banerjee, S. et al. (2011) Development 138:3287.
  7. Amenta, A.R. et al. (2012) J. Neurosci. 32:2324.
  8. Otsuka, K. et al. (2015) Scientific Reports 5:13928.
  9. Zhang, B. et al. (2008) Neuron 60:285.
  10. Yilmaz, A. et al. (2016) Science Signaling 9:ra87.
  11. Zhang, W. et al. (2011) J. Biol. Chem. 286:40624.
  12. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.
Long Name
Muscle-specific Receptor Tyrosine Kinase
Entrez Gene IDs
4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)
Alternate Names
EC 2.7.10; EC 2.7.10.1; MGC126323; MGC126324; muscle, skeletal, receptor tyrosine kinase; MuSK; skeletal receptor tyrosine-protein kinase

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