Recombinant Mouse Nectin-4 Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Tyr28-Ile349, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3116-N4
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Nectin-4
Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins (1). The Latin word necto means “to connect”, indicating the role of nectins in Ca2+‑independent cell-cell adhesion (2). Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4 (1 ‑ 3). Mouse Nectin-4 mRNA is found in the embryo, lung, testis and brain, while in the human it is normally expressed in the placenta, especially in endothelial cells (1, 4, 5). Mouse Nectin-4 cDNA encodes 508 amino acids (aa), including a 30 aa signal sequence, a 317 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type (3). One isoform lacks aa 410-434 in the cytoplasmic domain (1). In many human ductal breast or non-small cell lung carcinomas, Nectin-4 is up-regulated and a soluble 43 kDa form is found in the plasma (4 ‑ 6). This form is generated from the membrane protein via the action of TACE/ADAM-17 (6). The extracellular domain of mouse Nectin-4 shares 91%, 97%, 92%, 90% and 89% amino acid sequence homology with the corresponding regions of human, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins (1, 2). These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections (2, 7).
- Reymond, N. et al. (2001) J. Biol. Chem. 276:43205.
- Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
- Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
- Fabre-Lafay, S. et al. (2007) BMC Cancer 7:73.
- Takano, A. et al. (2009) Cancer Res. 69:6694.
- Fabre-Lafay, S. et al. (2005) J. Biol. Chem. 280:19543.
- Brancati, F. et al. (2010) Am. J. Hum. Genet. 87:265.
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