Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF
Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF Summary
Product Specifications
| MDP | Mouse IgG2A (Glu98-Lys330) | IEGR | Mouse NKG2A (Ala94-IIe227) Accession # NP_034782 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9330-NK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
When Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Chimera (Catalog # 9330-NK) is coated at 0.5 µg/mL, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED50 of 0.25-1.5 µg/mL.
Reconstitution Calculator
Background: NKG2A/NKG2B Isoform 2
NKG2B is a member of the C-type lectin superfamily of proteins. It is a splice variant of NKG2A which has an additional 17 aa in the extracellular juxtamembrane region (1). NKG2B is an approximately 40 kDa transmembrane protein, with 28 aa in the transmembrane region, and 105 aa in the extracellular domain (ECD). Within the ECD, mouse NKG2B shares 40% and 67% aa identity with human and rat NKG2B, respectively. NKG2B is expressed on a subset of NK cells and CD8+ T cells (2-6) where it forms a covalent heterodimer with CD94 (5, 7, 8). NKG2A-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (2, 7). Triggering the NKG2A-CD94 complex inhibits the cytolytic activity of NK and CD8+ T cells (2, 3, 5, 6, 9). This enables the innate immune system to detect cells that express host MHC-I molecules and to protect them from NK cell mediated lysis. This mechanism is subverted by human cytomegalovirus which encodes a peptide that is homologous to the HLA-E binding peptide (10). HCMV infected cells up-regulate both HLA-E and NKG2A expression and utilize this peptide to escape from immune clearance (3, 10). In contrast, vaccinia virus induces HLA-E down-regulation, thus permitting NK cell lysis of the virally infected cell (11).
- Lohwasser S, et al. (1999) Eur J Immunol. 29:755.
- Vance, R.E. et al. (1998) J. Exp. Med. 188:1841.
- Saez-Borderias, A. et al. (2009) J. Immunol.182:829.
- Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
- Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
- Zhou, J. et al. (2008) J. Immunol. 180:25.
- Braud, V.M. et al. (1998) Nature 391:795.
- Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
- Lee, N. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5199.
- Tomasec, P. et al. (2000) Science 287:1031.
- Brooks, C.R. et al. (2006) J. Immunol. 176:1141.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF and earn rewards!
Have you used Recombinant Mouse NKG2A/NKG2B Isoform 2 Fc Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
