Recombinant Mouse Notch-2 Fc Chimera Protein, CF

Catalog #: 5196-NT Datasheet / COA / SDS

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5196-NT-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Notch-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rmNotch-2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rrJagged-1/Fc Chimera with an apparent K_D < 5 nM.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Notch-2 protein

Mouse Notch-2 (Leu26 - Val528) Accession #NP_035058	IEGRMDP	Mouse IgG_2a (Glu98 - Lys330)
N-terminus		C-terminus

Accession #

NP_035058

N-terminal Sequence
Analysis

Leu26

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

80.8 kDa (monomer)

SDS-PAGE

105-120 kDa, reducing conditions

Product Datasheets

5196-NT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5196-NT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Notch-2

Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four Notch homologues involved in development (1 - 3). Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, demonstrating that not all functions overlap (4, 5). Mice with hypomorphic Notch-2 show defects in development of kidney, heart and eye (6). Notch-2 is upregulated in mature B cells and is critical for differentiation to splenic marginal zone B cells (7). Notch-2 is also preferentially expressed in choroid plexus epithelia and neuronal precursors (8, 9). Mouse Notch-2 is synthesized as a 2470 amino acid (aa) precursor that contains a 25 aa signal sequence, a 1652 aa extracellular domain (ECD), a 21 aa transmembrane (TM) segment, and a 772 aa cytoplasmic domain. The ECD contains 35 EGF-like repeats and three Lin-12/Notch repeats, while the cytoplasmic region shows six ankyrin repeats, a glutamine-rich domain and a PEST sequence. Binding of ligands, including Jagged and Delta-like molecules, has been localized to the 11^th and 12^th EGF-like repeats of human Notch-1 (corresponding to aa 413 - 492 in mouse Notch-2) (10). Notch receptors undergo post-translational furin-type proteolytic cleavage (11). This forms a heterodimer through the interaction of a hydrophobic area in the ligand-binding extracellular region with the TM/cytoplasmic portion (11, 12). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM-17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (13). Mouse Notch-2 ECD (aa 26 - 528) shares 93%, 96%, 92% and 92% aa identity with the corresponding regions of human, rat, canine, and bovine Notch-2, respectively. This region also exhibits 55 - 58% aa identity with mouse Notch-1 and Notch-3.

References

Lardelli, M. and U. Lendahl (1993) Exp. Cell Res. 204:364.
Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
Hamada, Y. et al. (1999) Development 126:3415.
Shimizu, K. et al. (2002) Biochem. Biophys. Res. Commun. 291:775.
McCright, B. et al. (2001) Development 128:491.
Saito, T. et al. (2003) Immunity 18:675.
Irvin, D. K. et al. (2001) J. Comp. Neurol. 436:167.
Solecki, D. J. et al. (2001) Neuron 31:557.
Hambleton, S. et al. (2004) Structure 12:2173.
Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Alternate Names

AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2