Home / Notum / Recombinant Mouse Notum Protein, CF

Recombinant Mouse Notum Protein, CF

Catalog #: 9150-NO
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9150-NO-050
Recombinant Mouse Notum Protein Bioactivity
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Notum Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.2-1.2  μg/mL in the presence of 0.1 μg/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Mouse myeloma cell line, NS0-derived mouse Notum protein
Ser88-Thr458 (Cys337Ser), with a C-terminal 6-His tag
Accession #
Q8R116
N-terminal Sequence
Analysis
Ser88, Ser239
Predicted Molecular Mass
43 kDa
SDS-PAGE
39-46 kDa, reducing conditions

Product Datasheets

You must select a language.

x

9150-NO

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9150-NO

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Notum Protein Bioactivity View Larger

Recombinant Mouse Wnt-3a shows a dose responsive increase in Wnt reporter activity in HEK293 cells (green line). Recombinant Mouse Notum (Catalog # 9150-NO) inhibits a constant dose of 0.1 µg/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 is 0.2-1.2 µg/mL (orange line).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Notum

Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids 88-458, mouse Notum shares 92% and 99% aa sequence identity with human and rat Notum, respectively.

References
  1. Zhang, X. et al. (2015) Dev. Cell 32:719.
  2. Kakugawa, S. et al. (2015) Nature 519:187.
  3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
  4. Traister, A. et al. (2008) Biochem. J. 410:503.
  5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
  6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
  7. De Robertis, M. et al. (2015) Oncotarget 6:41237.
Entrez Gene IDs
147111 (Human); 77583 (Mouse); 303743 (Rat)
Alternate Names
EC 3.-; notum pectinacetylesterase homolog (Drosophila); Notum; protein notum homolog

Citations for Recombinant Mouse Notum Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. 5-Aza-2'-Deoxycytidine Ameliorates Choroidal Neovascularization by Inhibiting the Wnt/?-Catenin Signaling Pathway
    Authors: Wu, X;Yang, X;Dai, X;Chen, X;Shen, M;Dai, J;Yuan, F;Wang, L;Yuan, Y;Feng, Y;
    Investigative ophthalmology & visual science
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Single-Cell Profiling and SCOPE-Seq Reveal Lineage Dynamics of Adult Ventricular-Subventricular Zone Neurogenesis and NOTUM as a Key Regulator
    Authors: D Mizrak, NS Bayin, J Yuan, Z Liu, RM Suciu, MJ Niphakis, N Ngo, KM Lum, BF Cravatt, AL Joyner, PA Sims
    Cell Rep, 2020-06-23;31(12):107805.
    Species: Mouse
    Sample Types: Organotypic Slices
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Mouse Notum Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Notum Protein, CF and earn rewards!

Have you used Recombinant Mouse Notum Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review