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Recombinant Mouse P-Selectin/CD62P Fc Chimera Protein, CF

Catalog #: 737-PS
27 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
737-PS-050
737-PS-200
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Citations (27)
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Recombinant Mouse P-Selectin/CD62P Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. P-Selectin/Fc Chimera immobilized at 10 µg/mL will induce greater than 50% adhesion on U937 cells.
Optimal dilutions should be determined be each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse P-Selectin/CD62P protein
Mouse P-Selectin
(Trp42-Ala709)
Accession # Q01102		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q01102
N-terminal Sequence
Analysis
Trp42
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
99.1 kDa (monomer)
SDS-PAGE
127-140 kDa, reducing conditions

Product Datasheets

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737-PS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

737-PS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P-Selectin/CD62P

Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

References
  1. Kansas, G.S. (1996) Blood 88:3259.
  2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

Citations for Recombinant Mouse P-Selectin/CD62P Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Dual phenotypic characteristics of P-selectin in a mouse model of hemorrhagic shock and hepatectomy
    Authors: Chen, JL;Cheng, TT;Huang, CC;Chang, HH;Lam, CF;
    Heliyon
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. A dhesion analysis via a tumor vasculature-like microfluidic device identifies CD8+ T�cells with enhanced tumor homing to improve cell therapy
    Authors: CP Camargo, AK Muhuri, Y Alapan, LF Sestito, M Khosla, MP Manspeaker, AS Smith, CM Paulos, SN Thomas
    Cell Reports, 2023-02-26;42(3):112175.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. A humanized beta2 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in�vivo
    Authors: L Wen, A Marki, Z Wang, M Orecchioni, J Makings, M Billitti, E Wang, SSA Suthahar, K Kim, WB Kiosses, Z Mikulski, K Ley
    Cell Reports, 2022-05-31;39(9):110876.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2
    Authors: MA Florez, KA Matatall, Y Jeong, L Ortinau, PW Shafer, AM Lynch, R Jaksik, M Kimmel, D Park, KY King
    Cell Reports, 2020-12-22;33(12):108530.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance
    Authors: V Barbier, J Erbani, C Fiveash, JM Davies, J Tay, MR Tallack, J Lowe, JL Magnani, DR Pattabiram, AC Perkins, J Lisle, JEJ Rasko, JP Levesque, IG Winkler
    Nat Commun, 2020-04-27;11(1):2042.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Targeted Expansion of Tissue-Resident CD8+ T Cells to Boost Cellular Immunity in the Skin
    Authors: SJ Hobbs, JC Nolz
    Cell Rep, 2019-12-03;29(10):2990-2997.e2.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus
    Authors: FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser
    Elife, 2018-09-06;7(0):.
  8. Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism
    Authors: Y Pan, T Tian, CO Park, SY Lofftus, S Mei, X Liu, C Luo, JT O'Malley, A Gehad, JE Teague, SJ Divito, R Fuhlbrigge, P Puigserver, JG Krueger, GS Hotamislig, RA Clark, TS Kupper
    Nature, 2017-02-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Dermal ?� T Cells Do Not Freely Re-Circulate Out of Skin and Produce IL-17 to Promote Neutrophil Infiltration during Primary Contact Hypersensitivity
    Authors: X Jiang, CO Park, J Geddes Swe, MJ Yoo, O Gaide, TS Kupper
    PLoS ONE, 2017-01-12;12(1):e0169397.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Sepsis-induced thymic atrophy is associated with defects in early lymphopoiesis
    Stem Cells, 2016-08-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Platelets Guide Leukocytes to Their Sites of Extravasation
    PLoS Biol, 2016-05-06;14(5):e1002459.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo.
    Authors: Velazquez F, Grodecki-Pena A, Knapp A, Salvador A, Nevers T, Croce K, Alcaide P
    J Immunol, 2015-12-23;196(3):1305-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin.
    Authors: Nussbaum C, Bannenberg S, Keul P, Graler M, Goncalves-de-Albuquerque C, Korhonen H, von Wnuck Lipinski K, Heusch G, de Castro Faria Neto H, Rohwedder I, Gothert J, Prasad V, Haufe G, Lange-Sperandio B, Offermanns S, Sperandio M, Levkau B
    Nat Commun, 2015-04-02;6(0):6416.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015-03-31;6(0):6387.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Raised soluble P-selectin moderately accelerates atherosclerotic plaque progression.
    Authors: Woollard K, Lumsden N, Andrews K, Aprico A, Harris E, Irvine J, Jefferis A, Fang L, Kanellakis P, Bobik A, Chin-Dusting J
    PLoS ONE, 2014-05-20;9(5):e97422.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Endothelial cell-derived chemerin promotes dendritic cell transmigration.
    Authors: Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S
    J Immunol, 2014-01-27;192(5):2366-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Difference in Th1 and Th17 lymphocyte adhesion to endothelium.
    Authors: Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
    J. Immunol., 2012-01-04;188(3):1421-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation.
    Authors: Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P
    J. Biol. Chem., 2011-09-12;286(44):38231-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  19. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  20. Kindlin-3 is required for beta2 integrin-mediated leukocyte adhesion to endothelial cells.
    Authors: Moser M, Bauer M, Schmid S, Ruppert R, Schmidt S, Sixt M, Wang HV, Sperandio M, Fassler R
    Nat. Med., 2009-02-22;15(3):300-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
    Authors: Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA
    Nat. Immunol., 2008-04-06;9(5):513-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression.
    Authors: Yamanaka K, Dimitroff CJ, Fuhlbrigge RC, Kakeda M, Kurokawa I, Mizutani H, Kupper TS
    J. Allergy Clin. Immunol., 2007-10-01;121(1):148-157.e3.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo.
    Authors: Alcaide P, King SL, Dimitroff CJ, Lim YC, Fuhlbrigge RC, Luscinskas FW
    J. Invest. Dermatol., 2007-03-29;127(8):1964-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Chondroitin sulfate glycosaminoglycans as major P-selectin ligands on metastatic breast cancer cell lines.
    Authors: Monzavi-Karbassi B, Stanley JS, Hennings L, Jousheghany F, Artaud C, Shaaf S, Kieber-Emmons T
    Int. J. Cancer, 2007-03-15;120(6):1179-91.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay, IHC-Fr
  25. Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells.
    Authors: Ni</LastName><ForeName>Zh Z</Initial, Ni Z, Walcheck B
    Immunol. Lett., 2007-01-03;108(2):179-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Dynamic programming of CD8+ T cell trafficking after live viral immunization.
    Authors: Liu L, Fuhlbrigge RC, Karibian K, Tian T, Kupper TS
    Immunity, 2006-09-01;25(3):511-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
    Authors: Cardones AR, Murakami T, Hwang ST
    Cancer Res., 2003-10-15;63(20):6751-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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