Recombinant Mouse Plexin B2 Protein, CF Summary
Product Specifications
When Recombinant Mouse Plexin B2 is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera (Catalog # 5235-S4) binds with an apparent KD < 10 nM.
Optimal dilutions should be determined by each laboratory for each applications.
| Mouse Plexin B2 (Leu20 - Trp1029) Accession # NP_001152993 |
HPGGGSGGGSGGGS | 6-His tag |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6836-PB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 400 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Plexin B2
Plexin B2 is a 240 kDa type I transmembrane (TM) glycoprotein of the Plexin B family of semaphorin receptors (1, 2). The mouse Plexin B2 cDNA encodes 1842 amino acids (aa) that include a 19 aa signal sequence, a 1182 aa extracellular domain (ECD), a 21 aa TM domain, and a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20 ‑ 468) and three IPT repeats (aa 806 ‑ 1096). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20 ‑ 1168) and an 80 kDa TM beta ‑chain, that remain noncovalently linked (1). Multiple splice variants may exist. Within aa 20 ‑ 1029 in the ECD, mouse Plexin B2 shares 82%, 93%, 80% and 79% aa identity with human, rat, canine and bovine Plexin B2, respectively. The B Plexins (B1, B2 and B3) share approximately 40% aa identity with each other. Plexin B2 mRNA is expressed in proliferating cerebellar granule cell progenitors, neuroepithelium, developing neurons, growth plate chondrocytes, tooth bud inner enamel epithelium, glomeruli and mesenchyme of the developing kidney, and in germinal center B lymphocytes when T cell help is present (3 ‑ 7). Plexin B2 is often co‑expressed with Plexin B1, and the two may form heterodimers (1, 4, 6). Genetic deletion of mouse Plexin B2 results in defects in proliferation and migration of cerebellar granule cells, abnormal development of the neural tube and disorganization of the embryonic brain; these defects are not seen when Plexin B1 is deleted (8 - 10). In adults, Plexin B2 is expressed in specialized vascular endothelia, pancreatic islets of Langerhans, and adrenal glands (11). Plexin B2 serves as a receptor for type 4 semaphorins, especially Sema4C and Sema4G (8 ‑ 12). B Plexins, including Plexin B2, can bind the scatter factor receptors, Met and Ron, and activate them upon semaphorin engagement (1, 13).
- Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
- Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
- Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
- Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
- Zhang, M. et al. (2008) Bone 43:511.
- Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
- Yu, D. et al. (2008) Immunol. Cell Biol. 86:3.
- Deng, S. et al. (2007) J. Neurosci. 27:6333.
- Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
- Maier, V. et al. (2011) Mol. Cell. Neurosci. 46:419.
- Zielonka, M. et al. (2010) Exp. Cell Res. 316:2477.
- Yukawa, K. et al. (2010) Int. J. Mol. Med. 25:225.
- Conrotto, P. et al. (2004) Oncogene 23:5131.
Citation for Recombinant Mouse Plexin B2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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In vivo interaction screening reveals liver-derived constraints to metastasis
Authors: Borrelli, C;Roberts, M;Eletto, D;Hussherr, MD;Fazilaty, H;Valenta, T;Lafzi, A;Kretz, JA;Guido Vinzoni, E;Karakatsani, A;Adivarahan, S;Mannhart, A;Kimura, S;Meijs, A;Baccouche Mhamedi, F;Acar, IE;Handler, K;Ficht, X;Platt, RJ;Piscuoglio, S;Moor, AE;
Nature
Species: Mouse
Sample Types: Organoid
Applications: Bioassay
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