Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF Summary
Product Specifications
Ala24-Gln305, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8545-PR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Pref-1/DLK1/FA1
Mouse Pref-1 (preadipocyte factor 1), also known as Dlk-1 or FA1, belongs to the Notch/Delta/Serrate family of EGF-like repeat-containing proteins that act as ligands in the Notch signaling pathway (1-3). Mouse Pref-1 is synthesized as a 385 amino acid (aa) precursor consisting of a 23 aa signal sequence, a 282 aa extracellular region, a 24 aa transmembrane segment, and a 56 aa cytoplasmic tail. The extracellular region contains six tandem EGF-like repeats, a juxtamembrane region, and multiple sites for O- and N-glycosylation. Heterogeneous transmembrane forms of Pref-1, ranging from 50 to 60 kDa, can be attributed to variability in glycosylation (2-4). In addition to full-length Pref-1A, alternative splicing of the juxtamembrane and EGF-like repeat regions generates three major short forms, Pref-1B, -1C, and
-1D (5). Proteolytic cleavage at one of two extracellular sites in Pref-1A and 1B can produce two soluble forms: a 50 kDa large form and a 24-25 kDa small form (2, 5, 6). Processing of Pref-1C and Pref-1D, by contrast, can produce only small soluble forms. Only the large soluble form demonstrates biological activity (5). The ECD of mature mouse Pref-1 shares 94% and 83% aa sequence identity with the ECD of human and rat Pref-1, respectively. Pref-1 is widely expressed during embryogenesis (2). In adults, Pref-1 expression is restricted to preadipocytes, pancreatic beta cells, thymic stromal cells, and cells of the adrenal and pituitary glands
(1, 7-9). Tumors of neuroendocrine origin have been found to express Pref-1 (10). Down-regulation of Pref-1 in preadipocytes is associated with differentiation into mature adipocytes (1-7). Pref-1 also regulates the differentiation of skeletal stem cells, thymocytes, and adrenal gland cells and inhibits hormone secretion in pituitary cells (1). Pref-1 has also been shown to inhibit preadipocyte proliferation (11, 12).
- Ansell, P.J. et al. (2007) Mol. Cell. Endocrinol. 271:55.
- Smas, C.M. and H.S. Sul (1993) Cell 73:725.
- Sul, H.S. (2009) Mol. Endocrinol. 23:1717.
- Smas, C.M. et al. (1994) Biochemistry 33:9257.
- Mei, B. et al. (2002) Biochem. J. 364:137.
- Wang, Y. and H.S. Sul (2006) Mol. Cell. Biol. 26:5421.
- Wang, Y. et al. (2010) Mol. Cell. Biol. 30:3480.
- Friedrichsen, B.N. et al. (2003) J. Endocrinol. 176:257.
- Hedlund, G.P. et al. (2003) In Vivo 17:1.
- Yin, D. et al. (2006) Oncogene 25:1852.
- Traustadottir, G.A. et al. (2013) Adipocyte 2:272.
- Mortensen, S.B. et al. (2012) Diabetes 61:2814.
FAQs
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What isoform is Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF (Catalog # 8545-PR) and what is the expected molecular weight?
This protein corresponds to the extracellular portion of Isoform A (Pref-1A). Isoforms B-D are short form isoforms generated from alternative splices of the immunogen sequence. This protein has an amino acid sequence of Ala24-Gln305 of Accession # Q09163, with a C-terminal 6-His tag and corresponds to the sequence of Isoform A. This sequence information can be found on UniProt under “Sequence” (https://www.uniprot.org/uniprot/Q09163#sequences). We would expect the protein to have a molecular weight between 49-66 kDa under reducing conditions.
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