Recombinant Mouse PSGL-1/CD162 Fc Chimera Protein, CF

Catalog #: 7407-PS
2 Citations Datasheet / COA / SDS

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7407-PS-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse PSGL-1/CD162 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED₅₀ for this effect is 0.15-0.6 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse PSGL-1/CD162 protein

Mouse PSGL-1 (Met1-Asp300) Accession # AAH03874	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

AAH03874

N-terminal Sequence
Analysis

Gln42

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

54 kDa (monomer)

SDS-PAGE

100-150 kDa, reducing conditions

Product Datasheets

7407-PS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7407-PS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: PSGL-1/CD162

PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a 110-120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). Mouse PSGL-1 cDNA encodes 397 amino acids (aa) including a 17 aa signal sequence, a 24 aa propeptide, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 69 aa cytoplasmic region (1). The ECD of mouse PSGL-1 contains 10 decameric aa repeats, or 12 repeats in a 417 aa long form, while human PSGL-1 contains 14-16 repeats (1, 3). Shorter forms may show weaker binding to P-selectin (9). Mouse PSGL-1 ECD shares 74% aa sequence identity with rat, and less than 40% with human, equine, canine and porcine PSGL-1 ECD. However, all share structural motifs and functions (1-3). The mature PSGL-1 (aa 42-397) is expressed as a disulfide-linked homodimer that signals intracellularly and promotes integrin activation (2-4). Ligand binding relies on presence of O-glycosylation/sialylated Lewis X (Le^X) and tyrosine sulfation within aa 42-57 (1, 5-7). Sulfation and glycosylation, which are constitutive on neutrophils and induced with activation on T cells, are essential for binding to P-selectin and L-selectin (3-6). E‑Selectin requires only glycosylation (3). Proteases such as neutrophil elastase and cathepsin G can cleave within this region, impacting binding affinity (3, 8). Cleaved, soluble ectodomains of PSGL-1 are also known (3). PSGL-1 is found on virtually all leukocytes, dendritic cells, platelets, and some endothelial cells (3). It is primarily responsible for early events in extravasation, especially rolling adhesion of leukocytes to vascular endothelium (3, 4). In addition, PGSL-1 binds chemokines such as CCL19, CCL21 and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs (3, 10, 11). Recombinant PSGL-1 ECD interferes with this chemotaxis (13). PSGL-1 binds von Willebrand factor in endothelial-derived Weibel-Palade bodies, and the chondroitin sulfate, versican (3). It is present with Tissue Factor in pro-thrombotic microparticles from membranes of monocytes and cancer cells, and is required for particle shedding and fusion with activated platelets (12, 13).

References

Yang, J. et al. (1999) Thromb. Haemost. 81:1.
Sako, D. et al. (1993) Cell 75:1179.
Carlow, D.A. et al. (2009) Immunol. Rev. 230:75.
Zarbock, A. et al. (2011) Blood 116:617.
Bernimoulin, M.P. et al. (2003) J. Biol. Chem. 278:37.
Sako, D. et al. (1995) Cell 83:323.
Xia, L. et al. (2003) Blood 101:552.
Gardiner, E.E. et al. (2001) Blood 98:1440.
Lozano, M.L. et al. (2001) Br. J. Haematol. 115:969.
Veerman, K.M. et al. (2007) Nat. Immunol. 8:532.
Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
del Conde, I. et al. (2005) Blood 106:1604.
Thomas, G.M. et al. (2009) J Exp. Med. 206:1913.

Long Name

P-Selectin Glycoprotein Ligand 1

Entrez Gene IDs

6404 (Human); 20345 (Mouse); 363930 (Rat)

Alternate Names

CD162 antigen; CD162; P-selectin glycoprotein ligand 1; PSGL1; PSGL-1; PSGL-1cutaneous lymphocyte-associated associated antigen; selectin P ligandCLA; SELPLG

Related Research Areas

Citations for Recombinant Mouse PSGL-1/CD162 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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